Brachymonas chironomi sp. nov., isolated from a chironomid egg mass, and emended description of the genus Brachymonas

Malka Halpern, Tamar Shakèd, Peter Schumann

Research output: Contribution to journalArticlepeer-review

Abstract

A Gram-negative, aerobic, chemo-organotrophic, catalase- and oxidase-positive strain, designated AIMA4T, was isolated from a chironomid (Diptera; Chironomidae) egg mass which was sampled from a waste stabilization pond in northern Israel. Phylogenetic analysis based on the 16S rRNA gene placed strain AIMA4T in the genus Brachymonas (class Betaproteobacteria). Strain AIMA4T shared 16S rRNA gene sequence similarity values of 96.9 % with Brachymonas denitrificans JCM 9216T and ≤95.7 % with the type strains of other genera. Strain AIMA4T was found to be a non-motile coccobacillus or rod that did not grow under anaerobic conditions and did not produce acid from carbohydrates. Growth was observed at salinities from 0 to 2.5 % (w/v) NaCl, at pH values from 5.0 to 9.0 and at temperatures from 18 to 37 °C. The major fatty acids (≥10 %) of strain AIMA4T were C16:ω7c, C16:0 and C18:1ω7c. Phosphatidylethanolamine, phosphatidylglycerol and phosphatidylserine were the predominant components of the polar lipids. The major ubiquinone was Q-8. On the basis of phenotypic properties and phylogenetic distinctiveness, strain AIMA4T (=LMG 24400T=DSM 19884T) was classified in the genus Brachymonas as the type strain of a novel species for which the name Brachymonas chironomi sp. nov. is proposed.

Original languageEnglish
Pages (from-to)3025-3029
Number of pages5
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume59
Issue number12
DOIs
StatePublished - 2009

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Microbiology

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