Bioinformatic identification of homing endonucleases and their target sites

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Homing endonuclease genes (HEGs) are a large, phylogenetically diverse superfamily of enzymes with high specificity for especially long target sites. The public genomic sequence databases contain thousands of HEGs. This is a large and diverse arsenal of potential genome editing tools. To make use of this natural resource, one needs to identify candidate HEGs. Due to their special relationship with a host gene, it is also possible to predict their cognate target sequences. Here I describe the HomeBase algorithm that was developed to this end. A detailed description of the computational pipeline is provided with emphasis on technical and methodological caveats of the approach.

Original languageEnglish
Title of host publicationHoming Endonucleases
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages27-35
Number of pages9
ISBN (Print)9781627039673
DOIs
StatePublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1123
ISSN (Print)1064-3745

Keywords

  • HomeBase
  • Homing endonucleases
  • Homology search
  • Target-site prediction

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint

Dive into the research topics of 'Bioinformatic identification of homing endonucleases and their target sites'. Together they form a unique fingerprint.

Cite this