Bidirectional Optogenetic Control of Inhibitory Neurons in Freely-Moving Mice

Ori Noked, Amir Levi, Shirly Someck, Ortal Amber-Vitos, Eran Stark

Research output: Contribution to journalArticlepeer-review


Objective: Optogenetic manipulations of excitable cells enable activating or silencing specific types of neurons. By expressing two types of exogenous proteins, a single neuron can be depolarized using light of one wavelength and hyperpolarized with another. However, routing two distinct wavelengths into the same brain locality typically requires bulky optics that cannot be implanted on the head of a freely-moving animal. Methods: We developed a lens-free approach for constructing dual-color head-mounted, fiber-based optical units: any two wavelengths can be combined. Results: Here, each unit was comprised of one 450 nm and one 638 nm laser diode, yielding light power of 0.4 mW and 8 mW at the end of a 50 micrometer multimode fiber. To create a multi-color/multi-site optoelectronic device, a four-shank silicon probe mounted on a microdrive was equipped with two dual-color and two single-color units, for a total weight under 3 g. Devices were implanted in mice expressing the blue-light sensitive cation channel ChR2 and the red-light sensitive chloride pump Jaws in parvalbumin-immunoreactive (PV) inhibitory neurons. The combination of dual-color units with recording electrodes was free from electromagnetic interference, and device heating was under 7°C even after prolonged operation. Conclusion: Using these devices, the same cortical PV cell could be activated and silenced. This was achieved for multiple cells both in neocortex and hippocampus of freely-moving mice. Significance: This technology can be used for controlling spatially intermingled neurons that have distinct genetic profiles, and for controlling spike timing of cortical neurons during cognitive tasks.

Original languageEnglish
Article number9112630
Pages (from-to)416-427
Number of pages12
JournalIEEE Transactions on Biomedical Engineering
Issue number2
StatePublished - Feb 2021
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 1964-2012 IEEE.


  • Electrophysiology
  • laser diodes
  • neural engineering
  • optoelectronic devices
  • sensor arrays

ASJC Scopus subject areas

  • Biomedical Engineering


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