Atrial natriuretic peptide induces acrosomal exocytosis in bovine spermatozoa

N. Zamir, D. Barkan, N. Keynan, Z. Naor, H. Breitbart

Research output: Contribution to journalArticlepeer-review

Abstract

The induction of acrosomal exocytosis in capacitated bull spermatozoa by atrial natriuretic peptide (ANP) was studied in vitro. ANP markedly stimulated acrosomal exocytosis in a calcium-dependent manner. Typically, ANP exerts its action via activation of the ANP receptor (ANPR-A), a particulate guanylyl cyclase-linked receptor, and subsequent formation of guanosine 3',5'-cyclic monophosphate (cGMP). We found that the ANP-induced acrosome reaction was inhibited by the competitive ANPR-A receptor antagonist-anantin, indicating a receptor-mediated effect. We could mimic the effect of ANP on the acrosome reaction by using 8-bromo-cGMP, suggesting that cGMP may serve as a signal transducer mediating the acrosome reaction. Indeed, the ANP- induced acrosome reaction was associated with elevation of cGMP levels. cGMP can also be formed by activation of the soluble form of guanylyl cyclase. Sodium nitroprusside (SNP) stimulated cGMP accumulation and acrosome reaction of capacitated spermatozoa. Thus ANP and the nitric oxide-releasing compound SNP, via activation of guanylyl cyclase (the former activating the particulate and the latter activating the soluble form of the enzyme), may play a significant role in the induction of the acrosome reaction.

Original languageEnglish
Pages (from-to)E216-E221
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume269
Issue number2 32-2
DOIs
StatePublished - 1995
Externally publishedYes

Keywords

  • acrosome reaction
  • guanosine 3',5'-cyclic monophosphate
  • guanylyl cyclase
  • spermatozoa

ASJC Scopus subject areas

  • Physiology (medical)
  • Physiology
  • Endocrinology, Diabetes and Metabolism

Fingerprint

Dive into the research topics of 'Atrial natriuretic peptide induces acrosomal exocytosis in bovine spermatozoa'. Together they form a unique fingerprint.

Cite this