Assessing expression of apoptotic markers using large cohort tissue microarrays

Elah Pick, Mary M. McCarthy, Harriet M. Kluger

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Apoptotic markers include proteins from the intrinsic and extrinsic pathways. These cascades include both pro-apoptotic and anti-apoptotic elements. The expression levels of these elements can be assessed by immunohistochemistry (IHC) and can indicate general trends in pro- versus anti-apoptotic tendencies of the cells. IHC is particularly useful when studying large cohorts of paraffin-embedded specimens. Advances in tissue microarray (TMA) technology have facilitated evaluation of large cohorts of specimens, as cores from hundreds of patients can be represented on a single glass slide and stained in a uniform fashion. In this chapter, we discuss construction and staining methods of TMAs and present examples of assessment of apoptotic marker expression in malignant and benign cells using a novel method of automated, quantitative analysis of in situ protein expression.

Original languageEnglish
Title of host publicationApoptosis and Cancer
Subtitle of host publicationMethods and Protocols
PublisherHumana Press
Pages83-93
Number of pages11
ISBN (Print)1597453390, 9781597453394
DOIs
StatePublished - 1 Dec 2007

Publication series

NameMethods in Molecular Biology
Volume414
ISSN (Print)1064-3745

Keywords

  • Cohort size
  • IHC
  • Immunofluorescence
  • Tissue microarrays

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

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