Assessing expression of apoptotic markers using large cohort tissue microarrays

Elah Pick, Mary M. McCarthy, Harriet M. Kluger

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


Apoptotic markers include proteins from the intrinsic and extrinsic pathways. These cascades include both pro-apoptotic and anti-apoptotic elements. The expression levels of these elements can be assessed by immunohistochemistry (IHC) and can indicate general trends in pro- versus anti-apoptotic tendencies of the cells. IHC is particularly useful when studying large cohorts of paraffin-embedded specimens. Advances in tissue microarray (TMA) technology have facilitated evaluation of large cohorts of specimens, as cores from hundreds of patients can be represented on a single glass slide and stained in a uniform fashion. In this chapter, we discuss construction and staining methods of TMAs and present examples of assessment of apoptotic marker expression in malignant and benign cells using a novel method of automated, quantitative analysis of in situ protein expression.

Original languageEnglish
Title of host publicationApoptosis and Cancer
Subtitle of host publicationMethods and Protocols
PublisherHumana Press
Number of pages11
ISBN (Print)1597453390, 9781597453394
StatePublished - 1 Dec 2007

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Cohort size
  • IHC
  • Immunofluorescence
  • Tissue microarrays

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology


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