Assessing expression of apoptotic markers using large cohort tissue microarrays

Elah Pick, Mary M. McCarthy, Harriet M. Kluger

    Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


    Apoptotic markers include proteins from the intrinsic and extrinsic pathways. These cascades include both pro-apoptotic and anti-apoptotic elements. The expression levels of these elements can be assessed by immunohistochemistry (IHC) and can indicate general trends in pro- versus anti-apoptotic tendencies of the cells. IHC is particularly useful when studying large cohorts of paraffin-embedded specimens. Advances in tissue microarray (TMA) technology have facilitated evaluation of large cohorts of specimens, as cores from hundreds of patients can be represented on a single glass slide and stained in a uniform fashion. In this chapter, we discuss construction and staining methods of TMAs and present examples of assessment of apoptotic marker expression in malignant and benign cells using a novel method of automated, quantitative analysis of in situ protein expression.

    Original languageEnglish
    Title of host publicationApoptosis and Cancer
    Subtitle of host publicationMethods and Protocols
    PublisherHumana Press
    Number of pages11
    ISBN (Print)1597453390, 9781597453394
    StatePublished - 1 Dec 2007

    Publication series

    NameMethods in Molecular Biology
    ISSN (Print)1064-3745


    • Cohort size
    • IHC
    • Immunofluorescence
    • Tissue microarrays

    ASJC Scopus subject areas

    • Molecular Biology
    • Genetics


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