@inbook{12d147347d4347e9b0fcd9a27adf9736,
title = "Assessing expression of apoptotic markers using large cohort tissue microarrays",
abstract = "Apoptotic markers include proteins from the intrinsic and extrinsic pathways. These cascades include both pro-apoptotic and anti-apoptotic elements. The expression levels of these elements can be assessed by immunohistochemistry (IHC) and can indicate general trends in pro- versus anti-apoptotic tendencies of the cells. IHC is particularly useful when studying large cohorts of paraffin-embedded specimens. Advances in tissue microarray (TMA) technology have facilitated evaluation of large cohorts of specimens, as cores from hundreds of patients can be represented on a single glass slide and stained in a uniform fashion. In this chapter, we discuss construction and staining methods of TMAs and present examples of assessment of apoptotic marker expression in malignant and benign cells using a novel method of automated, quantitative analysis of in situ protein expression.",
keywords = "Cohort size, IHC, Immunofluorescence, Tissue microarrays",
author = "Elah Pick and McCarthy, {Mary M.} and Kluger, {Harriet M.}",
year = "2007",
month = dec,
day = "1",
doi = "10.1385/1-59745-339-0:83",
language = "English",
isbn = "1597453390",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "83--93",
booktitle = "Apoptosis and Cancer",
address = "United States",
}