TY - JOUR
T1 - Araçatuba virus
T2 - A vaccinialike virus associated with infection in humans and cattle
AU - De Souza Trindade, Giliane
AU - Da Fonseca, Flávio Guimarães
AU - Marques, João Trindade
AU - Nogueira, Maurício Lacerda
AU - Mendes, Luiz Claudio Nogueira
AU - Borges, Alexandre Secorun
AU - Peiró, Juliana Regina
AU - Pituco, Edviges Maristela
AU - Bonjardim, Cláudio Antônio
AU - Ferreira, Paulo César Peregrino
AU - Kroon, Erna Geessien
PY - 2003/2/1
Y1 - 2003/2/1
N2 - We describe a vaccinialike virus, Araçatuba virus, associated with a cowpoxlike outbreak in a dairy herd and a related case of human infection. Diagnosis was based on virus growth characteristics, electron microscopy, and molecular biology techniques. Molecular characterization of the virus was done by using polymerase chain reaction amplification, cloning, and DNA sequencing of conserved orthopoxvirus genes such as the vaccinia growth factor (VGF), thymidine kinase (TK), and hemagglutinin. We used VGF-homologous and TK gene nucleotide sequences to construct a phylogenetic tree for comparison with other poxviruses. Gene sequences showed 99% homology with vaccinia virus genes and were clustered together with the isolated virus in the phylogenetic tree. Araçatuba virus is very similar to Cantagalo virus, showing the same signature deletion in the gene. Araçatuba virus could be a novel vaccinialike virus or could represent the spread of Cantagalo virus.
AB - We describe a vaccinialike virus, Araçatuba virus, associated with a cowpoxlike outbreak in a dairy herd and a related case of human infection. Diagnosis was based on virus growth characteristics, electron microscopy, and molecular biology techniques. Molecular characterization of the virus was done by using polymerase chain reaction amplification, cloning, and DNA sequencing of conserved orthopoxvirus genes such as the vaccinia growth factor (VGF), thymidine kinase (TK), and hemagglutinin. We used VGF-homologous and TK gene nucleotide sequences to construct a phylogenetic tree for comparison with other poxviruses. Gene sequences showed 99% homology with vaccinia virus genes and were clustered together with the isolated virus in the phylogenetic tree. Araçatuba virus is very similar to Cantagalo virus, showing the same signature deletion in the gene. Araçatuba virus could be a novel vaccinialike virus or could represent the spread of Cantagalo virus.
UR - http://www.scopus.com/inward/record.url?scp=0344211874&partnerID=8YFLogxK
U2 - 10.3201/eid0902.020244
DO - 10.3201/eid0902.020244
M3 - Article
C2 - 12603984
AN - SCOPUS:0344211874
SN - 1080-6040
VL - 9
SP - 155
EP - 160
JO - Emerging Infectious Diseases
JF - Emerging Infectious Diseases
IS - 2
ER -