Antioxidant activities of selected medicinal mushroom submerged cultivated mycelia

Yu Ming Liu, Yi Hsin Lai, Fu Mi Hsieh, Yi Min Chiu, Solomon P. Wasser, Jeng Leun Mau

Research output: Contribution to journalArticlepeer-review


Medicinal mushrooms fruiting bodies have been used as food or medicine for years but cultured myce-lium is faster to grow and costs less. This research studied the antioxidant activities of three species (five strains) of medicinal mushroom mycelia (Cordyceps militaris, Ganoderma tsugae I and II, Trametes versicolor I and II). Two-stage extractions were performed: first the sample was extracted with 70% ethanol, and then the residue was extracted with 95ºC hot water. Both ethanolic and hot water extracts showed effective concentration (EC50) values of 0.29–4.22 mg/mL, indicating that these extracts were remarkably effective in antioxidant activities. The ethanolic extracts dis-played more effective reducing power, scavenging, and chelating ability (EC50 0.33–2.37 mg/mL) than hot water extracts (EC50 0.58–4.22 mg/g). Besides, ethanolic extracts contained higher total phenol content (75.49–144.99 GAE mg/g) than the hot water extracts (22.77–58.68 GAE mg/g). Furthermore, the ethanolic extracts contained flavonoids but not the hot water extract. Overall, these mycelia were highly effective in the antioxidant activities and might be potent antioxidants.

Original languageEnglish
Pages (from-to)367-378
Number of pages11
JournalInternational Journal of Medicinal Mushrooms
Issue number4
StatePublished - 2020

Bibliographical note

Publisher Copyright:
© 2020 by Begell House, Inc.


  • Antioxidation
  • Cordyceps militaris
  • Ganoderma tsugae
  • Medicinal mushrooms
  • Mushroom mycelia
  • Trametes versicolor
  • Batch Cell Culture Techniques
  • Flavonoids/analysis
  • Ethanol/chemistry
  • Antioxidants/chemistry
  • Fungi/chemistry
  • Water/chemistry
  • Phenol/analysis
  • Mycelium/chemistry

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Drug Discovery
  • Pharmacology


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