Antimicrobial susceptibility of environmental non-O1/non-O139 vibrio cholerae isolates

Sivan Laviad-Shitrit, Yehonatan Sharaby, Ido Izhaki, Avi Peretz, Malka Halpern

Research output: Contribution to journalArticlepeer-review

Abstract

Vibrio cholerae serogroups O1 and O139 are the causative agents of cholera disease. There are more than 200 serogroups in this species that are termed V. cholerae non-O1/non-O139. Non-O1/non-O139 strains can cause gastroenteritis and cholera like diarrhea, wound infections, external otitis, and bacteraemia that may lead to mortality. Previous antimicrobial susceptibility studies were conducted mainly on O1/O139 serogroups and on clinical isolates. Our aim was to study and compare the antimicrobial susceptibilities of non-O1/non-O139 environmental strains isolated from chironomids, fish, and waterfowl. Significant differences were found in the antimicrobial susceptibilities between the environmental strains that were isolated from three different reservoir habitats. Significant increase in minimum inhibitory concentrations (MICs) of ampicillin and chloramphenicol was found in chironomid isolates from 2009 compared to those from 2005. V. cholerae isolates from different waterfowl species displayed the highest MIC values to chloramphenicol and trimethoprim-sulfamethoxazole (SXT), while chironomid isolates demonstrated the highest MIC values toward ampicillin. Isolates from fish and waterfowl showed high MIC values toward doxycycline. No significant differences were found between the MICs of isolates from the different waterfowl species. The percentage of antimicrobial resistance among V. cholerae isolates from waterfowl was the highest compared to the abundance of antimicrobial resistant isolates from chironomids or fish. The antimicrobial resistance genes can be carried on mobile genetic elements, thus, waterfowl may act as reservoirs for these elements and may spread them all over the globe. Data regarding treatment with antimicrobial agents toward V. cholerae non-O1/non-O139 serogroups is lacking and therefore further studies are needed.

Original languageEnglish
Article number1726
JournalFrontiers in Microbiology
Volume9
Issue numberAUG
DOIs
StatePublished - 2 Aug 2018

Bibliographical note

Funding Information:
This work was supported in part by a grant from the Israel Science Foundation (ISF Grant No. 296/16) and in part by the Binational Science Foundation (BSF Grant No. 2015103).

Publisher Copyright:
© 2018 Laviad-Shitrit, Sharaby, Izhaki, Peretz and Halpern.

Keywords

  • Antibiotics
  • Antimicrobial susceptibility
  • Chironomid
  • Fish
  • Resistance
  • V. cholerae
  • Waterbird
  • Waterfowl

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Fingerprint

Dive into the research topics of 'Antimicrobial susceptibility of environmental non-O1/non-O139 vibrio cholerae isolates'. Together they form a unique fingerprint.

Cite this