Ancient olive DNA in pits: Preservation, amplification and sequence analysis

Rivka Elbaum, Cathy Melamed-Bessudo, Elisabetta Boaretto, Ehud Galili, Simcha Lev-Yadun, Avraham A. Levy, Steve Weiner

Research output: Contribution to journalArticlepeer-review

Abstract

The olive tree (Olea europaea) was domesticated by vegetative propagation of selected wild individuals with superior fruit. Later, new cultivars were established repeatedly from feral trees or from crosses between wild, feral, and domesticated trees. Thus the genetic background of many contemporary domesticated lines is a mixture of ancient cultivars and local wild trees. Ancient DNA may illuminate the complicated process of olive domestication because such DNA sequences provide data about ancient genomes that existed closer to the domestication events. Well preserved DNA must be available for such studies, even though in the Mediterranean region, where olive cultivation took place, the climatic conditions are not favorable for DNA preservation. To select for well preserved pits we measured their proportions of lignin by IR spectroscopy, and correlated this with parameters of DNA quality such as template length in an olive-specific repeat array, and template quantity as determined by real-time PCR amplification. Archaeological pits that passed these tests did contain high quality ancient DNA. We present the first ancient olive DNA sequences and compare them to modern wild, feral and domesticated lines.

Original languageEnglish
Pages (from-to)77-88
Number of pages12
JournalJournal of Archaeological Science
Volume33
Issue number1
DOIs
StatePublished - Jan 2006
Externally publishedYes

Bibliographical note

Funding Information:
We thank Yuval Peleg for supplying the olive pits from Qumran, Dr. Bengamin Avidan for supplying modern olive leaves and pits, Michal Salamon for the information about DNA preservation in relation to collagen preservation in archaeological human bones, and Prof. Angela Schlumbaum for analyzing aDNA from 2 of the waterlogged olives in her lab. This study was funded in part by generous support from Mr. George Schwartzmann, Sarasota, Florida, by the BIKURA foundation, and by the Kimmel Center for Archaeological Science at the Weizmann Institute. S.W. holds the Dr. Walter and Dr. Trude Borchardt Professorial Chair in Structural Biology.

Keywords

  • aDNA
  • Infrared
  • ITS1
  • Lignin
  • OeGEM86 repeat
  • Olea europaea
  • TrnT-L spacer

ASJC Scopus subject areas

  • Archaeology
  • Archaeology

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