Analysis and validation of genome-specific DNA variations in 5′ flanking conserved sequences of wheat low-molecular-weight glutenin subunit genes

Hai Long, Yuming Wei, Zehong Yan, Bernard Baum, Eviatar Nevo, Youliang Zheng

Research output: Contribution to journalArticlepeer-review

Abstract

The thirty-three 5′ flanking conserved sequences of the known low-molecular-weight subunit (LMW-GS) genes have been divided into eight clusters, which was in agreement with the classification based on the deduced N-terminal protein sequences. The DNA polymorphism between the eight clusters was obtained by sequence alignment, and a total of 34 polymorphic positions were observed in the approximately 200 bp regions, among which 18 polymorphic positions were candidate SNPs. Seven cluster-specific primer sets were designed for seven out of eight clusters containing cluster-specific bases, with which the genomic DNA of the ditelosomic lines of group 1 chromosomes of a wheat variety 'Chinese Spring' was employed to carry out chromosome assignment. The subsequent cloning and DNA sequencing of PCR fragments validated the sequences specificity of the 5′ flanking conserved sequences between LMW-GS gene groups in different genomes. These results suggested that the coding and 5′ flanking regions of LMW-GS genes are likely to have evolved in a concerted fashion. The seven primer sets developed in this study could be used to isolate the complete ORFs of seven groups of LMW-GS genes, respectively, and therefore possess great value for further research in the contributions of a single LMW-GS gene to wheat quality in the complex genetic background and the efficient selections of quality-related components in breeding programs.

Original languageEnglish
Pages (from-to)322-331
Number of pages10
JournalScience in China, Series C: Life Sciences
Volume49
Issue number4
DOIs
StatePublished - Aug 2006

Bibliographical note

Funding Information:
Acknowledgements This work was supported by the National High Technology Research and Development Program of China (863 Program) (Grant No. 2003AA207100), National Natural Science Foundation of China (Grant Nos. 30300219 & 30571163), and the Foundation for the Author of National Excellent Doctoral Dissertation of China from Ministry of Education, China (Grant Nos. 200357 & 200458). Zheng Youliang was supported by the Program for Changjiang Scholars and Innovative Research Teams in University of China (Grant No. IRT0453)

Keywords

  • 5′ flanking sequence
  • DNA polymorphism
  • Gene cloning
  • LMW-GS gene
  • SNP
  • Wheat

ASJC Scopus subject areas

  • General Agricultural and Biological Sciences
  • General Environmental Science
  • Energy Engineering and Power Technology
  • General Energy
  • General Biochemistry, Genetics and Molecular Biology
  • Management, Monitoring, Policy and Law
  • Fuel Technology
  • Renewable Energy, Sustainability and the Environment
  • Modeling and Simulation

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