Drought limits plant growth and threatens crop productivity. A barley (Hordeum vulgare) ethylene imine-induced monogenic recessive mutant cer-zv, which is sensitive to drought, was characterized and genetically mapped in the present study. Detached leaves of cer-zv lost 34. 2 % of their initial weight after 1 h of dehydration. The transpiration was much higher in cer-zv leaves than in wild-type leaves under both light and dark conditions. The stomata of cer-zv leaves functioned normally, but the cuticle of cer-zv leaves showed increased permeability to ethanol and toluidine blue dye. There was a 50-90 % reduction in four major cutin monomers, but no reduction in wax loads was found in the cer-zv mutant as compared with the wild type. Two F2 mapping populations were established by the crosses of 23-19 × cer-zv and cer-zv × OUH602. More polymorphisms were found in EST sequences between cer-zv and OUH602 than between cer-zv and 23-19. cer-zv was located in a pericentromeric region on chromosome 4H in a 10. 8 cM interval in the 23-19 × cer-zv map based on 186 gametes tested and a 1. 7 cM interval in the cer-zv × OUH602 map based on 176 gametes tested. It co-segregated with EST marker AK251484 in both maps. The results indicated that the cer-zv mutant is defective in cutin, which might be responsible for the increased transpiration rate and drought sensitivity, and that the F2 of cer-zv × OUH602 might better facilitate high resolution mapping of cer-zv.
Bibliographical noteFunding Information:
We thank Drs. Y. Nagamura, J. Song and Mrs. Y. Ma for their helpful and constructive contributions. We thank Mrs. R. Permut for English editing. This research was supported by the One Hundred Talents Project of the Chinese Academy of Sciences (O827751001) and the National Natural Science Foundation of China (30970449, 31170369, and 31160036), and Genomics for Agricultural Innovation of the Ministry of Agriculture, Forestry, and Fisheries of Japan (TRG1007), as well as by the Swiss National Science Foundation (grant 31003A-125009 to C.N.).
ASJC Scopus subject areas
- Agronomy and Crop Science