TY - JOUR
T1 - An ATP-binding cassette subfamily G full transporter is essential for the retention of leaf water in both wild barley and rice
AU - Chen, Guoxiong
AU - Komatsuda, Takao
AU - Ma, Jian Feng
AU - Nawrath, Christiane
AU - Pourkheirandish, Mohammad
AU - Tagiri, Akemi
AU - Hu, Yin Gang
AU - Sameri, Mohammad
AU - Li, Xinrong
AU - Zhao, Xin
AU - Liu, Yubing
AU - Li, Chao
AU - Ma, Xiaoying
AU - Wang, Aidong
AU - Nair, Sudha
AU - Wang, Ning
AU - Miyao, Akio
AU - Sakuma, Shun
AU - Yamaji, Naoki
AU - Zheng, Xiuting
AU - Nevo, Eviatar
PY - 2011/7/26
Y1 - 2011/7/26
N2 - Land plants have developed a cuticle preventing uncontrolled water loss. Here we report that an ATP-binding cassette (ABC) subfamily G (ABCG) full transporter is required for leaf water conservation in both wild barley and rice. A spontaneous mutation, eibi1.b, in wild barley has a low capacity to retain leaf water, a phenotype associated with reduced cutin deposition and a thin cuticle. Map-based cloning revealed that Eibi1 encodes an HvABCG31 full transporter. The gene was highly expressed in the elongation zone of a growing leaf (the site of cutin synthesis), and its gene product also was localized in developing, but not in mature tissue. A de novo wild barley mutant named "eibi1.c," along with two transposon insertion lines of rice mutated in the ortholog of HvABCG31 also were unable to restrict water loss from detached leaves. HvABCG31 is hypothesized to function as a transporter involved in cutin formation. Homologs of HvABCG31 were found in green algae, moss, and lycopods, indicating that this full transporter is highly conserved in the evolution of land plants.
AB - Land plants have developed a cuticle preventing uncontrolled water loss. Here we report that an ATP-binding cassette (ABC) subfamily G (ABCG) full transporter is required for leaf water conservation in both wild barley and rice. A spontaneous mutation, eibi1.b, in wild barley has a low capacity to retain leaf water, a phenotype associated with reduced cutin deposition and a thin cuticle. Map-based cloning revealed that Eibi1 encodes an HvABCG31 full transporter. The gene was highly expressed in the elongation zone of a growing leaf (the site of cutin synthesis), and its gene product also was localized in developing, but not in mature tissue. A de novo wild barley mutant named "eibi1.c," along with two transposon insertion lines of rice mutated in the ortholog of HvABCG31 also were unable to restrict water loss from detached leaves. HvABCG31 is hypothesized to function as a transporter involved in cutin formation. Homologs of HvABCG31 were found in green algae, moss, and lycopods, indicating that this full transporter is highly conserved in the evolution of land plants.
KW - Cuticular wax
KW - Drought resistance
KW - Pleiotropic drug resistance
KW - Radiation mutagenesis
KW - Transposon Oryza sativa 17
UR - http://www.scopus.com/inward/record.url?scp=79961078109&partnerID=8YFLogxK
U2 - 10.1073/pnas.1108444108
DO - 10.1073/pnas.1108444108
M3 - Article
C2 - 21737747
AN - SCOPUS:79961078109
SN - 0027-8424
VL - 108
SP - 12354
EP - 12359
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 30
ER -