An approach for high sensitivity detection of prostate cancer by analysis of changes in structuredness of the cytoplasmic matrix of lymphocytes specifically induced by PSA-ACT

Ofer Klein, Shai Lin, Oscar Embon, Alejandro Sazbon, Jamal Zidan, Abraham I. Kook

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Purpose: An alternative procedure for detection of prostate cancer was examined based on the observation that cells reexposed in vitro to antigenic or mitogenic stimulation will change their intracellular structuredness as measured by polarization of fluorescent light emitted by labeled cells (SCM test). Materials and Methods: Lymphocytes derived from patients bearing a nonmalignant prostate tumor and healthy individuals were exposed to PSA-ACT, PHA, and MUC-1. Results: Of sixty-five patients with prostate carcinoma (CAP), sixty-two were correctly diagnosed by the test. Of the eighty males in the control group, five were incorrectly diagnosed as having the disease and seventy-five were correctly diagnosed as healthy subjects. The sensitivity of the test was 96.8%. The specificity was 91.1%. The BPH (Benign Prostatic Hyperplasia) control group exhibited a sensitivity of 9.38%, but the specificity was 91.1%. Similar percentages for specificity and sensitivity were observed in the NRT (Non-Relevant Tumor) control group. Conclusions: The results shown here indicate the possibility of a different use of PSA-ACT for detection of prostate cancer with high specificity and sensitivity.

    Original languageEnglish
    Pages (from-to)1994-1996
    Number of pages3
    JournalJournal of Urology
    Volume161
    Issue number6
    DOIs
    StatePublished - Jun 1999

    Keywords

    • Prostate cancer
    • Prostate specific antigen

    ASJC Scopus subject areas

    • Urology

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