Alterations in delayed and direct phytoplankton fluorescence in response to the diurnal light cycle

Eyal Kurzbaum; Sven Beer; Werner Eckert

doi:10.1007/s10750-009-0024-2

Alterations in delayed and direct phytoplankton fluorescence in response to the diurnal light cycle

Eyal Kurzbaum, Sven Beer, Werner Eckert

Research output: Contribution to journal › Article › peer-review

Abstract

Delayed fluorescence (DF) excitation spectrometry was examined as a proxy for phytoplankton activity in comparison to pulse-amplitude-modulated (PAM) fluorometry and dissolved oxygen (DO) evolution. During several day-night cycles, the three target variables were monitored simultaneously, together with pH, temperature and photosynthetically active irradiance in an exponentially growing Chlorella population exposed to natural light conditions. It was found that during a diel cycle prompt and DF signals corresponded to each other and were negatively correlated to the light intensity, with maximum values during night and vice versa. The DF signal showed a strong linear relationship with the quantum yield of photosystem II. Our findings thus suggest that, in addition to the continuous monitoring of active chlorophyll of different taxonomical groups, DF excitation spectrometry also carries the potential to continuously monitor the quantum yields and relative electron transport rates in natural phytoplankton assemblages.

Original language	English
Pages (from-to)	197-203
Number of pages	7
Journal	Hydrobiologia
Volume	639
Issue number	1
DOIs	https://doi.org/10.1007/s10750-009-0024-2
State	Published - Jan 2010
Externally published	Yes

Bibliographical note

Funding Information:
Acknowledgements E. K. thanks the ‘‘Yochai Ben Nun Scholarship’’ from the Israel Oceanographic and Limnological Research Ltd. for support during this work. Fruitful discussions and technical assistance by V. Gerhardt and U. Bodemer are gratefully acknowledged. We wish to thank A. Sukenik and Y.Z. Yacobi as well as two anonymous reviewers for their critical comments of the manuscript. This study was presented as a contributed paper at the Bat Sheva de Rothschild seminar on Phytoplankton in the Physical Environment—the 15th workshop of the International Association of Phytoplankton Taxonomy and Ecology (IAP).

Keywords

Chlorella
Delayed fluorescence
PAM fluorometer
Photosynthesis
Phytoplankton
Prompt fluorescence
Quantum yield

ASJC Scopus subject areas

Aquatic Science

Access to Document

10.1007/s10750-009-0024-2

Cite this

@article{b8f7b425db8546739cc41b58cfe3cdb2,

title = "Alterations in delayed and direct phytoplankton fluorescence in response to the diurnal light cycle",

abstract = "Delayed fluorescence (DF) excitation spectrometry was examined as a proxy for phytoplankton activity in comparison to pulse-amplitude-modulated (PAM) fluorometry and dissolved oxygen (DO) evolution. During several day-night cycles, the three target variables were monitored simultaneously, together with pH, temperature and photosynthetically active irradiance in an exponentially growing Chlorella population exposed to natural light conditions. It was found that during a diel cycle prompt and DF signals corresponded to each other and were negatively correlated to the light intensity, with maximum values during night and vice versa. The DF signal showed a strong linear relationship with the quantum yield of photosystem II. Our findings thus suggest that, in addition to the continuous monitoring of active chlorophyll of different taxonomical groups, DF excitation spectrometry also carries the potential to continuously monitor the quantum yields and relative electron transport rates in natural phytoplankton assemblages.",

keywords = "Chlorella, Delayed fluorescence, PAM fluorometer, Photosynthesis, Phytoplankton, Prompt fluorescence, Quantum yield",

author = "Eyal Kurzbaum and Sven Beer and Werner Eckert",

note = "Funding Information: Acknowledgements E. K. thanks the {\textquoteleft}{\textquoteleft}Yochai Ben Nun Scholarship{\textquoteright}{\textquoteright} from the Israel Oceanographic and Limnological Research Ltd. for support during this work. Fruitful discussions and technical assistance by V. Gerhardt and U. Bodemer are gratefully acknowledged. We wish to thank A. Sukenik and Y.Z. Yacobi as well as two anonymous reviewers for their critical comments of the manuscript. This study was presented as a contributed paper at the Bat Sheva de Rothschild seminar on Phytoplankton in the Physical Environment—the 15th workshop of the International Association of Phytoplankton Taxonomy and Ecology (IAP).",

year = "2010",

month = jan,

doi = "10.1007/s10750-009-0024-2",

language = "English",

volume = "639",

pages = "197--203",

journal = "Hydrobiologia",

issn = "0018-8158",

publisher = "Springer Netherlands",

number = "1",

}

TY - JOUR

T1 - Alterations in delayed and direct phytoplankton fluorescence in response to the diurnal light cycle

AU - Kurzbaum, Eyal

AU - Beer, Sven

AU - Eckert, Werner

N1 - Funding Information: Acknowledgements E. K. thanks the ‘‘Yochai Ben Nun Scholarship’’ from the Israel Oceanographic and Limnological Research Ltd. for support during this work. Fruitful discussions and technical assistance by V. Gerhardt and U. Bodemer are gratefully acknowledged. We wish to thank A. Sukenik and Y.Z. Yacobi as well as two anonymous reviewers for their critical comments of the manuscript. This study was presented as a contributed paper at the Bat Sheva de Rothschild seminar on Phytoplankton in the Physical Environment—the 15th workshop of the International Association of Phytoplankton Taxonomy and Ecology (IAP).

PY - 2010/1

Y1 - 2010/1

N2 - Delayed fluorescence (DF) excitation spectrometry was examined as a proxy for phytoplankton activity in comparison to pulse-amplitude-modulated (PAM) fluorometry and dissolved oxygen (DO) evolution. During several day-night cycles, the three target variables were monitored simultaneously, together with pH, temperature and photosynthetically active irradiance in an exponentially growing Chlorella population exposed to natural light conditions. It was found that during a diel cycle prompt and DF signals corresponded to each other and were negatively correlated to the light intensity, with maximum values during night and vice versa. The DF signal showed a strong linear relationship with the quantum yield of photosystem II. Our findings thus suggest that, in addition to the continuous monitoring of active chlorophyll of different taxonomical groups, DF excitation spectrometry also carries the potential to continuously monitor the quantum yields and relative electron transport rates in natural phytoplankton assemblages.

AB - Delayed fluorescence (DF) excitation spectrometry was examined as a proxy for phytoplankton activity in comparison to pulse-amplitude-modulated (PAM) fluorometry and dissolved oxygen (DO) evolution. During several day-night cycles, the three target variables were monitored simultaneously, together with pH, temperature and photosynthetically active irradiance in an exponentially growing Chlorella population exposed to natural light conditions. It was found that during a diel cycle prompt and DF signals corresponded to each other and were negatively correlated to the light intensity, with maximum values during night and vice versa. The DF signal showed a strong linear relationship with the quantum yield of photosystem II. Our findings thus suggest that, in addition to the continuous monitoring of active chlorophyll of different taxonomical groups, DF excitation spectrometry also carries the potential to continuously monitor the quantum yields and relative electron transport rates in natural phytoplankton assemblages.

KW - Chlorella

KW - Delayed fluorescence

KW - PAM fluorometer

KW - Photosynthesis

KW - Phytoplankton

KW - Prompt fluorescence

KW - Quantum yield

UR - http://www.scopus.com/inward/record.url?scp=75849161093&partnerID=8YFLogxK

U2 - 10.1007/s10750-009-0024-2

DO - 10.1007/s10750-009-0024-2

M3 - Article

AN - SCOPUS:75849161093

SN - 0018-8158

VL - 639

SP - 197

EP - 203

JO - Hydrobiologia

JF - Hydrobiologia

IS - 1

ER -

Alterations in delayed and direct phytoplankton fluorescence in response to the diurnal light cycle

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this