AFLP genetic polymorphism in wild barley (Hordeum spontaneum) populations in Israel

T. Turpeinen, T. Vanhala, E. Nevo, E. Nissilä

Research output: Contribution to journalArticlepeer-review

Abstract

The genetic diversity produced by the amplified fragment length polymorphism (AFLP) method was studied in 94 genotypes of wild barley, Hordeum spontaneum (C. Koch) Thell., originating from ten ecologically and geographically different locations in Israel. Eight primer pairs produced 204 discernible loci of which 189 (93%) were polymorphic. Each genotype had a unique banding profile and the genetic similarity coefficient varied between 0.74 and 0.98. The phenogram generated from these similarities by the UPGMA method did not group genotypes strictly according to their geographical origin, which pattern was also seen in the principal coordinate (PCO) plot. Genetic diversity was larger within (69%) than among (31%) populations. Associations between ecogeographical variables and the mean gene diversity were found at one primer pair. The results are discussed and compared with data obtained by the simple sequence repeat (SSR) method.

Original languageEnglish
Pages (from-to)1333-1339
Number of pages7
JournalTheoretical And Applied Genetics
Volume106
Issue number7
DOIs
StatePublished - 1 May 2003

Keywords

  • AFLP
  • Amplified fragment length polymorphism
  • Genetic diversity
  • Hordeum spontaneum
  • Wild barley

ASJC Scopus subject areas

  • Genetics
  • Agronomy and Crop Science
  • Biotechnology

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