Abstract
To study the regulation of expression of the Serratia plymuthica gene chiA encoding a 58-kDa endochitinase, its 586-bp-long upstream regulatory region was cloned, sequenced and fused to a promoterless lac operon in phage λRS45 to obtain a single-copy transcriptional fusion (PF1chiA-lac) in lysogens of Escherichia coli wild-type strains or their mutants deficient in various global regulators of transcription. The level of PF1chiA-lac expression increased about 20- and 90-fold, respectively, in E. coli K12 Δhns and double Δhns stpA mutants deficient in H-NS, and in both H-NS and StpA DNA-binding histone-like proteins, as compared to levels in the wild-type strain. In a Δlrp mutant deficient in the leucine-responsive transcriptional regulator Lrp, the level of PF1chiA-lac expression increased only up to threefold, whereas even smaller differences relative to the wild-type strain were observed in rpoS and Δcrp mutants deficient in the σS subunit of RNA polymerase and catabolite-repression protein (CRP), respectively. Deletion of the inverted-repeat sequences and curved DNA regions located in the upstream region of chiA essentially did not influence strain IC1270's chiA promoter activity in E. coli.
Original language | English |
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Pages (from-to) | 426-437 |
Number of pages | 12 |
Journal | Journal of Basic Microbiology |
Volume | 45 |
Issue number | 6 |
DOIs | |
State | Published - 2005 |
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology