Activation of EphB2 Forward Signaling Enhances Memory Consolidation

EphB2 is involved in enhancing synaptic transmission and gene expression. To explore the roles of EphB2 in memory formation and enhancement, we used a photoactivatable EphB2 (optoEphB2) to activate EphB2 forward signaling in pyramidal neurons in lateral amygdala (LA). Photoactivation of optoEphB2 during fear conditioning, but not minutes afterward, enhanced long-term, but not short-term, auditory fear conditioning. Photoactivation of optoEphB2 during fear conditioning led to activation of the cAMP/Ca²⁺ responsive element binding (CREB) protein. Application of light to a kinase-dead optoEphB2 in LA did not lead to enhancement of long-term fear conditioning memory or to activation of CREB. Long-term, but not short-term, auditory fear conditioning memory was impaired in mice lacking EphB2 forward signaling (EphB2^lacZ/lacZ). Activation of optoEphB2 in LA of EphB2^lacZ/lacZ mice enhanced long-term fear conditioning memory. The present findings show that the level of EphB2 forward signaling activity during learning determines the strength of long-term memory consolidation. Alapin et al. show that activation of photoactivatable EphB2 (optoEphB2) in the lateral amygdala of mice during fear conditioning specifically enhances long-term auditory fear conditioning memory and CREB activation. However, the kinase-dead opto-EphB2 has no effects on LTM or pCREB. Photoactivation of optoEphB2 in LA of EphB2^lacZ/lacZ mice enhances fear LTM.