Acceleration of leaf senescence is slowed down in transgenic barley plants deficient in the DNA/RNA-binding protein WHIRLY1

Weronika Kucharewicz; Assaf Distelfeld; Wolfgang Bilger; Maren Müller; Sergi Munné-Bosch; Götz Hensel; Karin Krupinska

doi:10.1093/jxb/erw501

Acceleration of leaf senescence is slowed down in transgenic barley plants deficient in the DNA/RNA-binding protein WHIRLY1

Weronika Kucharewicz, Assaf Distelfeld, Wolfgang Bilger, Maren Müller, Sergi Munné-Bosch, Götz Hensel, Karin Krupinska

Research output: Contribution to journal › Article › peer-review

Abstract

WHIRLY1 in barley was isolated as a potential regulator of the senescence-associated gene HvS40. In order to investigate whether the plastid-nucleus-located DNA/RNA-binding protein WHIRLY1 plays a role in regulation of leaf senescence, primary foliage leaves from transgenic barley plants with an RNAi-mediated knockdown of the WHIRLY1 gene were characterized by typical senescence parameters, namely pigment contents, function and composition of the photosynthetic apparatus, as well as expression of selected genes known to be either down- or up-regulated during leaf senescence. When the plants were grown at low light intensity, senescence progression was similar between wild-type and RNAi-W1 plants. Likewise, dark-induced senescence of detached leaves was not affected by reduction of WHIRLY1. When plants were grown at high light intensity, however, senescence was induced prematurely in wildtype plants but was delayed in RNAi-W1 plants. This result suggests that WHIRLY1 plays a role in light sensing and/or stress communication between chloroplasts and the nucleus.

Original language	English
Pages (from-to)	983-996
Number of pages	14
Journal	Journal of Experimental Botany
Volume	68
Issue number	5
DOIs	https://doi.org/10.1093/jxb/erw501
State	Published - 1 Feb 2017
Externally published	Yes

Bibliographical note

Funding Information:
We acknowledge the excellent technical assistance of Susanne Braun and Ulrike Voigt (Institute of Botany, CAU Kiel, Germany). Hartmut Kaiser is thanked for the setting up of gas exchange measurements. We also acknowledge the initial investigations performed by Dr Elzbieta Golemic. We thank Poul-Erik Jensen, University of Copenhagen, Denmark, for providing the antibody directed towards photosystem I proteins. WK was supported by the European Marie Curie Initial Training Network 'CropLife'. KK and AD acknowledge support from the German-Israeli Foundation for Scientific Research and Development (GIF) grant no. I-1212-315.13.

Publisher Copyright:
© The Author 2017.

Keywords

Barley
Chloroplast proteins
DNA/RNA-binding protein WHIRLY1
High light
Leaf senescence
Stay-green

ASJC Scopus subject areas

Physiology
Plant Science

Access to Document

10.1093/jxb/erw501

Cite this

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title = "Acceleration of leaf senescence is slowed down in transgenic barley plants deficient in the DNA/RNA-binding protein WHIRLY1",

abstract = "WHIRLY1 in barley was isolated as a potential regulator of the senescence-associated gene HvS40. In order to investigate whether the plastid-nucleus-located DNA/RNA-binding protein WHIRLY1 plays a role in regulation of leaf senescence, primary foliage leaves from transgenic barley plants with an RNAi-mediated knockdown of the WHIRLY1 gene were characterized by typical senescence parameters, namely pigment contents, function and composition of the photosynthetic apparatus, as well as expression of selected genes known to be either down- or up-regulated during leaf senescence. When the plants were grown at low light intensity, senescence progression was similar between wild-type and RNAi-W1 plants. Likewise, dark-induced senescence of detached leaves was not affected by reduction of WHIRLY1. When plants were grown at high light intensity, however, senescence was induced prematurely in wildtype plants but was delayed in RNAi-W1 plants. This result suggests that WHIRLY1 plays a role in light sensing and/or stress communication between chloroplasts and the nucleus.",

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author = "Weronika Kucharewicz and Assaf Distelfeld and Wolfgang Bilger and Maren M{\"u}ller and Sergi Munn{\'e}-Bosch and G{\"o}tz Hensel and Karin Krupinska",

note = "Funding Information: We acknowledge the excellent technical assistance of Susanne Braun and Ulrike Voigt (Institute of Botany, CAU Kiel, Germany). Hartmut Kaiser is thanked for the setting up of gas exchange measurements. We also acknowledge the initial investigations performed by Dr Elzbieta Golemic. We thank Poul-Erik Jensen, University of Copenhagen, Denmark, for providing the antibody directed towards photosystem I proteins. WK was supported by the European Marie Curie Initial Training Network 'CropLife'. KK and AD acknowledge support from the German-Israeli Foundation for Scientific Research and Development (GIF) grant no. I-1212-315.13. Publisher Copyright: {\textcopyright} The Author 2017.",

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AU - Müller, Maren

AU - Munné-Bosch, Sergi

AU - Hensel, Götz

AU - Krupinska, Karin

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Acceleration of leaf senescence is slowed down in transgenic barley plants deficient in the DNA/RNA-binding protein WHIRLY1

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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