Absorption pathways of amino acids in the midgut of Manduca sexta larvae

Moshe Reuveni; Peter E. Dunn

doi:10.1016/0965-1748(93)90113-7

Absorption pathways of amino acids in the midgut of Manduca sexta larvae

Research output: Contribution to journal › Article › peer-review

Abstract

The unidirectional transport of leucine and aspartic acid by midgut epithelium cells of the tobacco ornworm, Manduca sexta, was investigated using brush border membrane vesicles. Both K⁺-stimulated and K⁺-insensitive transport pathways for these two amino acids were identified. Kinetic analyses and profiles of cis-inhibition by other amino acids demonstrated that leucine and aspartic acid are transported by separate pathways and exhibit different sensitivity to potassium ion.

Original language	English
Pages (from-to)	959-966
Number of pages	8
Journal	Insect Biochemistry and Molecular Biology
Volume	23
Issue number	8
DOIs	https://doi.org/10.1016/0965-1748(93)90113-7
State	Published - Dec 1993
Externally published	Yes

Bibliographical note

Funding Information:
Several pathways that transport different amino acids, such as phenylalanine, histidine, glutamic acid, lysine and alanine, have been described in midgut brush border membrane vesicles (BBMV) from Pieris brassicae *This research was supported by USDA Competitive Research Grant No. 88-37153-4098 to P. E. Dunn. This is journal paper No. 12641 from the Indiana Agricultural Experiment Station. tDepartment of Entomology,1 158 EntomologyH all, Purdue Univer-sity, West Lafayette,I N 47907-1158, U.S.A. :~Author for correspondence.

Keywords

Amino acid transport
Brush border membrane vesicle
Manduca sexta
Midgut epithelium

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Insect Science

Access to Document

10.1016/0965-1748(93)90113-7

Cite this

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title = "Absorption pathways of amino acids in the midgut of Manduca sexta larvae",

abstract = "The unidirectional transport of leucine and aspartic acid by midgut epithelium cells of the tobacco ornworm, Manduca sexta, was investigated using brush border membrane vesicles. Both K+-stimulated and K+-insensitive transport pathways for these two amino acids were identified. Kinetic analyses and profiles of cis-inhibition by other amino acids demonstrated that leucine and aspartic acid are transported by separate pathways and exhibit different sensitivity to potassium ion.",

keywords = "Amino acid transport, Brush border membrane vesicle, Manduca sexta, Midgut epithelium",

author = "Moshe Reuveni and Dunn, {Peter E.}",

note = "Funding Information: Several pathways that transport different amino acids, such as phenylalanine, histidine, glutamic acid, lysine and alanine, have been described in midgut brush border membrane vesicles (BBMV) from Pieris brassicae *This research was supported by USDA Competitive Research Grant No. 88-37153-4098 to P. E. Dunn. This is journal paper No. 12641 from the Indiana Agricultural Experiment Station. tDepartment of Entomology,1 158 EntomologyH all, Purdue Univer-sity, West Lafayette,I N 47907-1158, U.S.A. :~Author for correspondence.",

year = "1993",

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doi = "10.1016/0965-1748(93)90113-7",

language = "English",

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AU - Reuveni, Moshe

AU - Dunn, Peter E.

N1 - Funding Information: Several pathways that transport different amino acids, such as phenylalanine, histidine, glutamic acid, lysine and alanine, have been described in midgut brush border membrane vesicles (BBMV) from Pieris brassicae *This research was supported by USDA Competitive Research Grant No. 88-37153-4098 to P. E. Dunn. This is journal paper No. 12641 from the Indiana Agricultural Experiment Station. tDepartment of Entomology,1 158 EntomologyH all, Purdue Univer-sity, West Lafayette,I N 47907-1158, U.S.A. :~Author for correspondence.

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Absorption pathways of amino acids in the midgut of Manduca sexta larvae

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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