A versatile plasmid biosensor useful to identify quorum sensing LuxR-family orphans in bacterial strains

Laura Steindler, Giulia Devescovi, Sujatha Subramoni, Vittorio Venturi

Research output: Contribution to journalArticlepeer-review

Abstract

Eight luxI-family gene promoters (luxI, cviI, ahlI, rhlI, cepI, phzI, traI and ppuI) were cloned in tandem, upstream a promoterless lacZ gene in a promoter probe vector yielding pMULTIAHLPROM. This unique construct is useful in determining whether a bacterial strain not producing N-acyl homoserine lactone signal molecules (AHLs) possesses orphan LuxR type proteins able to respond to AHLs and activate transcription from quorum sensing target genes. Using pMULTIAHLPROM, it was demonstrated that Enterobacter aerogenes possibly contains a LuxR-family orphan able to activate luxI-family promoters independently from AHLs.

Original languageEnglish
Pages (from-to)273-275
Number of pages3
JournalJournal of Microbiological Methods
Volume73
Issue number3
DOIs
StatePublished - Jun 2008
Externally publishedYes

Bibliographical note

Funding Information:
L.S. and S.S. are supported by an ICGEB fellowship. V.V.'s laboratory in the ICGEB Biosafety Outstation in Ca'Tron is also supported by the Fondazione Cassamarca, Treviso, Italy.

Keywords

  • Biosensor
  • LuxR
  • N-acyl homoserine lactone
  • Plasmid
  • Quorum sensing

ASJC Scopus subject areas

  • Microbiology (medical)
  • Molecular Biology
  • Microbiology

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