A versatile plasmid biosensor useful to identify quorum sensing LuxR-family orphans in bacterial strains

Laura Steindler; Giulia Devescovi; Sujatha Subramoni; Vittorio Venturi

doi:10.1016/j.mimet.2008.02.022

A versatile plasmid biosensor useful to identify quorum sensing LuxR-family orphans in bacterial strains

Laura Steindler, Giulia Devescovi, Sujatha Subramoni, Vittorio Venturi

Research output: Contribution to journal › Article › peer-review

Abstract

Eight luxI-family gene promoters (luxI, cviI, ahlI, rhlI, cepI, phzI, traI and ppuI) were cloned in tandem, upstream a promoterless lacZ gene in a promoter probe vector yielding pMULTIAHLPROM. This unique construct is useful in determining whether a bacterial strain not producing N-acyl homoserine lactone signal molecules (AHLs) possesses orphan LuxR type proteins able to respond to AHLs and activate transcription from quorum sensing target genes. Using pMULTIAHLPROM, it was demonstrated that Enterobacter aerogenes possibly contains a LuxR-family orphan able to activate luxI-family promoters independently from AHLs.

Original language	English
Pages (from-to)	273-275
Number of pages	3
Journal	Journal of Microbiological Methods
Volume	73
Issue number	3
DOIs	https://doi.org/10.1016/j.mimet.2008.02.022
State	Published - Jun 2008
Externally published	Yes

Bibliographical note

Funding Information:
L.S. and S.S. are supported by an ICGEB fellowship. V.V.'s laboratory in the ICGEB Biosafety Outstation in Ca'Tron is also supported by the Fondazione Cassamarca, Treviso, Italy.

Keywords

Biosensor
LuxR
N-acyl homoserine lactone
Plasmid
Quorum sensing

ASJC Scopus subject areas

Microbiology
Molecular Biology
Microbiology (medical)

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10.1016/j.mimet.2008.02.022

Cite this

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title = "A versatile plasmid biosensor useful to identify quorum sensing LuxR-family orphans in bacterial strains",

abstract = "Eight luxI-family gene promoters (luxI, cviI, ahlI, rhlI, cepI, phzI, traI and ppuI) were cloned in tandem, upstream a promoterless lacZ gene in a promoter probe vector yielding pMULTIAHLPROM. This unique construct is useful in determining whether a bacterial strain not producing N-acyl homoserine lactone signal molecules (AHLs) possesses orphan LuxR type proteins able to respond to AHLs and activate transcription from quorum sensing target genes. Using pMULTIAHLPROM, it was demonstrated that Enterobacter aerogenes possibly contains a LuxR-family orphan able to activate luxI-family promoters independently from AHLs.",

keywords = "Biosensor, LuxR, N-acyl homoserine lactone, Plasmid, Quorum sensing",

author = "Laura Steindler and Giulia Devescovi and Sujatha Subramoni and Vittorio Venturi",

note = "Funding Information: L.S. and S.S. are supported by an ICGEB fellowship. V.V.'s laboratory in the ICGEB Biosafety Outstation in Ca'Tron is also supported by the Fondazione Cassamarca, Treviso, Italy. ",

year = "2008",

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doi = "10.1016/j.mimet.2008.02.022",

language = "English",

volume = "73",

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AU - Steindler, Laura

AU - Devescovi, Giulia

AU - Subramoni, Sujatha

AU - Venturi, Vittorio

N1 - Funding Information: L.S. and S.S. are supported by an ICGEB fellowship. V.V.'s laboratory in the ICGEB Biosafety Outstation in Ca'Tron is also supported by the Fondazione Cassamarca, Treviso, Italy.

PY - 2008/6

Y1 - 2008/6

N2 - Eight luxI-family gene promoters (luxI, cviI, ahlI, rhlI, cepI, phzI, traI and ppuI) were cloned in tandem, upstream a promoterless lacZ gene in a promoter probe vector yielding pMULTIAHLPROM. This unique construct is useful in determining whether a bacterial strain not producing N-acyl homoserine lactone signal molecules (AHLs) possesses orphan LuxR type proteins able to respond to AHLs and activate transcription from quorum sensing target genes. Using pMULTIAHLPROM, it was demonstrated that Enterobacter aerogenes possibly contains a LuxR-family orphan able to activate luxI-family promoters independently from AHLs.

AB - Eight luxI-family gene promoters (luxI, cviI, ahlI, rhlI, cepI, phzI, traI and ppuI) were cloned in tandem, upstream a promoterless lacZ gene in a promoter probe vector yielding pMULTIAHLPROM. This unique construct is useful in determining whether a bacterial strain not producing N-acyl homoserine lactone signal molecules (AHLs) possesses orphan LuxR type proteins able to respond to AHLs and activate transcription from quorum sensing target genes. Using pMULTIAHLPROM, it was demonstrated that Enterobacter aerogenes possibly contains a LuxR-family orphan able to activate luxI-family promoters independently from AHLs.

KW - Biosensor

KW - LuxR

KW - N-acyl homoserine lactone

KW - Plasmid

KW - Quorum sensing

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A versatile plasmid biosensor useful to identify quorum sensing LuxR-family orphans in bacterial strains

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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