A sterol-defined system for quantitative studies of sterol metabolism in C. elegans

Benjamin Trabelcy; Yoram Gerchman; Amir Sapir

doi:10.1016/j.xpro.2021.100710

A sterol-defined system for quantitative studies of sterol metabolism in C. elegans

Benjamin Trabelcy, Yoram Gerchman, Amir Sapir

Department of Biology and Environment (Oranim Campus)

Research output: Contribution to journal › Article › peer-review

Abstract

This protocol describes the culturing of the nematode Caenorhabditis elegans (C. elegans) in a sterol-defined experimental system and the subsequent quantitative analysis of C. elegans sterols through gas chromatography-mass spectrometry. Although studied primarily in mammals, sterols are essential biomolecules for most eukaryotes. C. elegans cannot synthesize sterols and thus relies on the uptake of dietary sterols. Therefore, C. elegans is a powerful system to study metabolism in sterol-defined conditions that are described in our protocol. For complete details on the use and execution of this protocol, please refer to Shamsuzzama et al. (2020).

Original language	English
Article number	100710
Journal	STAR Protocols
Volume	2
Issue number	3
DOIs	https://doi.org/10.1016/j.xpro.2021.100710
State	Published - 17 Sep 2021

Bibliographical note

Publisher Copyright:
© 2021 The Authors

Keywords

Genetics
Mass Spectrometry
Metabolism
Model Organisms

ASJC Scopus subject areas

General Neuroscience
General Biochemistry, Genetics and Molecular Biology
General Immunology and Microbiology

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10.1016/j.xpro.2021.100710

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abstract = "This protocol describes the culturing of the nematode Caenorhabditis elegans (C. elegans) in a sterol-defined experimental system and the subsequent quantitative analysis of C. elegans sterols through gas chromatography-mass spectrometry. Although studied primarily in mammals, sterols are essential biomolecules for most eukaryotes. C. elegans cannot synthesize sterols and thus relies on the uptake of dietary sterols. Therefore, C. elegans is a powerful system to study metabolism in sterol-defined conditions that are described in our protocol. For complete details on the use and execution of this protocol, please refer to Shamsuzzama et al. (2020).",

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AB - This protocol describes the culturing of the nematode Caenorhabditis elegans (C. elegans) in a sterol-defined experimental system and the subsequent quantitative analysis of C. elegans sterols through gas chromatography-mass spectrometry. Although studied primarily in mammals, sterols are essential biomolecules for most eukaryotes. C. elegans cannot synthesize sterols and thus relies on the uptake of dietary sterols. Therefore, C. elegans is a powerful system to study metabolism in sterol-defined conditions that are described in our protocol. For complete details on the use and execution of this protocol, please refer to Shamsuzzama et al. (2020).

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KW - Metabolism

KW - Model Organisms

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A sterol-defined system for quantitative studies of sterol metabolism in C. elegans

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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