A sterol-defined system for quantitative studies of sterol metabolism in C. elegans

Benjamin Trabelcy; Yoram Gerchman; Amir Sapir

doi:10.1016/j.xpro.2021.100710

A sterol-defined system for quantitative studies of sterol metabolism in C. elegans

Benjamin Trabelcy, Yoram Gerchman, Amir Sapir

Faculty of Natural Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

This protocol describes the culturing of the nematode Caenorhabditis elegans (C. elegans) in a sterol-defined experimental system and the subsequent quantitative analysis of C. elegans sterols through gas chromatography-mass spectrometry. Although studied primarily in mammals, sterols are essential biomolecules for most eukaryotes. C. elegans cannot synthesize sterols and thus relies on the uptake of dietary sterols. Therefore, C. elegans is a powerful system to study metabolism in sterol-defined conditions that are described in our protocol. For complete details on the use and execution of this protocol, please refer to Shamsuzzama et al. (2020).

Original language	English
Article number	100710
Journal	STAR Protocols
Volume	2
Issue number	3
DOIs	https://doi.org/10.1016/j.xpro.2021.100710
State	Published - 17 Sep 2021

Bibliographical note

Funding Information:
Some strains were provided by the Caenorhabditis Genetics Center, which is funded by the National Institute of Health Office of Research Infrastructure Programs ( P40OD010440 ). This work was supported by an Israel Science Foundation grant ( 1747/16 ) to A.S.

Publisher Copyright:
© 2021 The Authors

Keywords

Genetics
Mass Spectrometry
Metabolism
Model Organisms

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology (all)
Immunology and Microbiology (all)
Neuroscience (all)

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10.1016/j.xpro.2021.100710

Cite this

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abstract = "This protocol describes the culturing of the nematode Caenorhabditis elegans (C. elegans) in a sterol-defined experimental system and the subsequent quantitative analysis of C. elegans sterols through gas chromatography-mass spectrometry. Although studied primarily in mammals, sterols are essential biomolecules for most eukaryotes. C. elegans cannot synthesize sterols and thus relies on the uptake of dietary sterols. Therefore, C. elegans is a powerful system to study metabolism in sterol-defined conditions that are described in our protocol. For complete details on the use and execution of this protocol, please refer to Shamsuzzama et al. (2020).",

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A sterol-defined system for quantitative studies of sterol metabolism in C. elegans

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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