A sterol-defined system for quantitative studies of sterol metabolism in C. elegans

Benjamin Trabelcy, Yoram Gerchman, Amir Sapir

Research output: Contribution to journalArticlepeer-review

Abstract

This protocol describes the culturing of the nematode Caenorhabditis elegans (C. elegans) in a sterol-defined experimental system and the subsequent quantitative analysis of C. elegans sterols through gas chromatography-mass spectrometry. Although studied primarily in mammals, sterols are essential biomolecules for most eukaryotes. C. elegans cannot synthesize sterols and thus relies on the uptake of dietary sterols. Therefore, C. elegans is a powerful system to study metabolism in sterol-defined conditions that are described in our protocol. For complete details on the use and execution of this protocol, please refer to Shamsuzzama et al. (2020).

Original languageEnglish
Article number100710
JournalSTAR Protocols
Volume2
Issue number3
DOIs
StatePublished - 17 Sep 2021

Bibliographical note

Publisher Copyright:
© 2021 The Authors

Keywords

  • Genetics
  • Mass Spectrometry
  • Metabolism
  • Model Organisms

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology
  • General Immunology and Microbiology
  • General Neuroscience

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