Abstract
DNA extraction of thraustochytrids, common marine unicellular organisms, is usually accomplished by either the cetyltrimethylammonium bromide (CTAB) or proteinase K protocols. A novel lysis buffer protocol for thraustochytrid total DNA extraction is described. The average isolated total DNA is 20 to 40 kb, and DNA samples are suitable for a variety of uses including 18S-ribosomal DNA polymerase chain reaction, restriction enzyme digestions, and amplified fragment length polymorphism analyses. The new protocol is also faster than the other protocols.
| Original language | English |
|---|---|
| Pages (from-to) | 100-102 |
| Number of pages | 3 |
| Journal | Marine Biotechnology |
| Volume | 3 |
| Issue number | 2 |
| DOIs | |
| State | Published - 2001 |
| Externally published | Yes |
Keywords
- DNA extraction
- Lysis buffer protocol
- Polymerase chain reaction
- Thraustochytrids
ASJC Scopus subject areas
- Biotechnology
- Aquatic Science
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