A simple, reliable, and fast protocol for thraustochytrid DNA extraction

Caiqing Mo, Baruch Rinkevich

Research output: Contribution to journalArticlepeer-review


DNA extraction of thraustochytrids, common marine unicellular organisms, is usually accomplished by either the cetyltrimethylammonium bromide (CTAB) or proteinase K protocols. A novel lysis buffer protocol for thraustochytrid total DNA extraction is described. The average isolated total DNA is 20 to 40 kb, and DNA samples are suitable for a variety of uses including 18S-ribosomal DNA polymerase chain reaction, restriction enzyme digestions, and amplified fragment length polymorphism analyses. The new protocol is also faster than the other protocols.

Original languageEnglish
Pages (from-to)100-102
Number of pages3
JournalMarine Biotechnology
Issue number2
StatePublished - 2001
Externally publishedYes


  • DNA extraction
  • Lysis buffer protocol
  • Polymerase chain reaction
  • Thraustochytrids

ASJC Scopus subject areas

  • Biotechnology
  • Aquatic Science


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