A simple, reliable, and fast protocol for thraustochytrid DNA extraction

Caiqing Mo; Baruch Rinkevich

doi:10.1007/s101260000069

A simple, reliable, and fast protocol for thraustochytrid DNA extraction

Research output: Contribution to journal › Article › peer-review

Abstract

DNA extraction of thraustochytrids, common marine unicellular organisms, is usually accomplished by either the cetyltrimethylammonium bromide (CTAB) or proteinase K protocols. A novel lysis buffer protocol for thraustochytrid total DNA extraction is described. The average isolated total DNA is 20 to 40 kb, and DNA samples are suitable for a variety of uses including 18S-ribosomal DNA polymerase chain reaction, restriction enzyme digestions, and amplified fragment length polymorphism analyses. The new protocol is also faster than the other protocols.

Original language	English
Pages (from-to)	100-102
Number of pages	3
Journal	Marine Biotechnology
Volume	3
Issue number	2
DOIs	https://doi.org/10.1007/s101260000069
State	Published - 2001
Externally published	Yes

Keywords

DNA extraction
Lysis buffer protocol
Polymerase chain reaction
Thraustochytrids

ASJC Scopus subject areas

Biotechnology
Aquatic Science

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10.1007/s101260000069

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title = "A simple, reliable, and fast protocol for thraustochytrid DNA extraction",

abstract = "DNA extraction of thraustochytrids, common marine unicellular organisms, is usually accomplished by either the cetyltrimethylammonium bromide (CTAB) or proteinase K protocols. A novel lysis buffer protocol for thraustochytrid total DNA extraction is described. The average isolated total DNA is 20 to 40 kb, and DNA samples are suitable for a variety of uses including 18S-ribosomal DNA polymerase chain reaction, restriction enzyme digestions, and amplified fragment length polymorphism analyses. The new protocol is also faster than the other protocols.",

keywords = "DNA extraction, Lysis buffer protocol, Polymerase chain reaction, Thraustochytrids",

author = "Caiqing Mo and Baruch Rinkevich",

year = "2001",

doi = "10.1007/s101260000069",

language = "English",

volume = "3",

pages = "100--102",

journal = "Marine Biotechnology",

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T1 - A simple, reliable, and fast protocol for thraustochytrid DNA extraction

AU - Mo, Caiqing

AU - Rinkevich, Baruch

PY - 2001

Y1 - 2001

N2 - DNA extraction of thraustochytrids, common marine unicellular organisms, is usually accomplished by either the cetyltrimethylammonium bromide (CTAB) or proteinase K protocols. A novel lysis buffer protocol for thraustochytrid total DNA extraction is described. The average isolated total DNA is 20 to 40 kb, and DNA samples are suitable for a variety of uses including 18S-ribosomal DNA polymerase chain reaction, restriction enzyme digestions, and amplified fragment length polymorphism analyses. The new protocol is also faster than the other protocols.

AB - DNA extraction of thraustochytrids, common marine unicellular organisms, is usually accomplished by either the cetyltrimethylammonium bromide (CTAB) or proteinase K protocols. A novel lysis buffer protocol for thraustochytrid total DNA extraction is described. The average isolated total DNA is 20 to 40 kb, and DNA samples are suitable for a variety of uses including 18S-ribosomal DNA polymerase chain reaction, restriction enzyme digestions, and amplified fragment length polymorphism analyses. The new protocol is also faster than the other protocols.

KW - DNA extraction

KW - Lysis buffer protocol

KW - Polymerase chain reaction

KW - Thraustochytrids

UR - http://www.scopus.com/inward/record.url?scp=0034814424&partnerID=8YFLogxK

U2 - 10.1007/s101260000069

DO - 10.1007/s101260000069

M3 - Article

AN - SCOPUS:0034814424

SN - 1436-2228

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JO - Marine Biotechnology

JF - Marine Biotechnology

IS - 2

ER -

A simple, reliable, and fast protocol for thraustochytrid DNA extraction

Abstract

Keywords

ASJC Scopus subject areas

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