A program which determines mutagenic concentrations of chemical carcinogens via a diffusion bioassay

Tamara E. Awerbuch; Elizabeth F. Ryder; David Faraggi

doi:10.1016/0169-2607(87)90075-7

A program which determines mutagenic concentrations of chemical carcinogens via a diffusion bioassay

Tamara E. Awerbuch, Elizabeth F. Ryder, David Faraggi

Research output: Contribution to journal › Article › peer-review

Abstract

A computer program implementing a mathematical model for determining mutagenic concentrations of chemical carcinogens was developed. The mathematical model describes the experiment in which a droplet of a suspected carcinogen is put at the center of a petri dish containing a bacterial lawn in an agar gel. After a period of incubation during which the chemical diffuses outward, one observes a concentric ring of mutants around the center. The largest radius at which mutation occurs, r_mut, corresponds to the lowest (threshold) concentration of the chemical sufficient to produce bacterial mutation. Given a series of initial concentrations of a chemical and the resulting r_muts, the program computes and reports the threshold concentration and the decay time of the chemical. The program is also used as a method to determine the lowest mutagenic concentration for a particular time of exposure.

Original language	English
Pages (from-to)	31-38
Number of pages	8
Journal	Computer Methods and Programs in Biomedicine
Volume	25
Issue number	1
DOIs	https://doi.org/10.1016/0169-2607(87)90075-7
State	Published - Aug 1987
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by a grant from the National Institute of Health: NIH R23 CA 37820.

Keywords

Ames test
Chemical carcinogens
Computer modeling
Diffusion bioassay

ASJC Scopus subject areas

Software
Computer Science Applications
Health Informatics

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10.1016/0169-2607(87)90075-7

Cite this

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title = "A program which determines mutagenic concentrations of chemical carcinogens via a diffusion bioassay",

abstract = "A computer program implementing a mathematical model for determining mutagenic concentrations of chemical carcinogens was developed. The mathematical model describes the experiment in which a droplet of a suspected carcinogen is put at the center of a petri dish containing a bacterial lawn in an agar gel. After a period of incubation during which the chemical diffuses outward, one observes a concentric ring of mutants around the center. The largest radius at which mutation occurs, rmut, corresponds to the lowest (threshold) concentration of the chemical sufficient to produce bacterial mutation. Given a series of initial concentrations of a chemical and the resulting rmuts, the program computes and reports the threshold concentration and the decay time of the chemical. The program is also used as a method to determine the lowest mutagenic concentration for a particular time of exposure.",

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author = "Awerbuch, {Tamara E.} and Ryder, {Elizabeth F.} and David Faraggi",

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doi = "10.1016/0169-2607(87)90075-7",

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AU - Awerbuch, Tamara E.

AU - Ryder, Elizabeth F.

AU - Faraggi, David

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AB - A computer program implementing a mathematical model for determining mutagenic concentrations of chemical carcinogens was developed. The mathematical model describes the experiment in which a droplet of a suspected carcinogen is put at the center of a petri dish containing a bacterial lawn in an agar gel. After a period of incubation during which the chemical diffuses outward, one observes a concentric ring of mutants around the center. The largest radius at which mutation occurs, rmut, corresponds to the lowest (threshold) concentration of the chemical sufficient to produce bacterial mutation. Given a series of initial concentrations of a chemical and the resulting rmuts, the program computes and reports the threshold concentration and the decay time of the chemical. The program is also used as a method to determine the lowest mutagenic concentration for a particular time of exposure.

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A program which determines mutagenic concentrations of chemical carcinogens via a diffusion bioassay

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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