A program which determines mutagenic concentrations of chemical carcinogens via a diffusion bioassay

Tamara E. Awerbuch, Elizabeth F. Ryder, David Faraggi

Research output: Contribution to journalArticlepeer-review

Abstract

A computer program implementing a mathematical model for determining mutagenic concentrations of chemical carcinogens was developed. The mathematical model describes the experiment in which a droplet of a suspected carcinogen is put at the center of a petri dish containing a bacterial lawn in an agar gel. After a period of incubation during which the chemical diffuses outward, one observes a concentric ring of mutants around the center. The largest radius at which mutation occurs, rmut, corresponds to the lowest (threshold) concentration of the chemical sufficient to produce bacterial mutation. Given a series of initial concentrations of a chemical and the resulting rmuts, the program computes and reports the threshold concentration and the decay time of the chemical. The program is also used as a method to determine the lowest mutagenic concentration for a particular time of exposure.

Original languageEnglish
Pages (from-to)31-38
Number of pages8
JournalComputer Methods and Programs in Biomedicine
Volume25
Issue number1
DOIs
StatePublished - Aug 1987
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported by a grant from the National Institute of Health: NIH R23 CA 37820.

Keywords

  • Ames test
  • Chemical carcinogens
  • Computer modeling
  • Diffusion bioassay

ASJC Scopus subject areas

  • Software
  • Health Informatics
  • Computer Science Applications

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