Here we describe a protein product of the human septin H5/PNUTL2/CDCrel2b gene, which we call ARTS (for apoptosis-related protein in the TGF-β signalling pathway). ARTS is expressed in many ceils and acts to enhance cell death induced by TGF-β or, to a lesser extent, by other apoptotic agents. Unlike related septin gene products, ARTS is localized to mitochondria and translocates to the nucleus when apoptosis occurs. Mutation of the P-loop of ARTS abrogates its competence to activate caspase 3 and to induce apoptosis. Taken together, these observations expand the functional attributes of septins previously described as having roles in cytokinesis and cellular morphogenesis.
Bibliographical noteFunding Information:
Ben-Neriah, A. Yaron, S. Gutkind and M. Zohar for discussions and advice; G. Yaniv and O. Binah for providing the neonatal ventricular myocytes; and N. Frumkin and A. H. Hsing for technical assistance. We thank D. B. Hales and K. H. Hales for providing the antiserum to recombinant murine StAR; the AU5–tag expression vector was provided by S. Gutkind; TGF-b receptor constructs were provided by J. Wrana and L. Attisano; and the p3TP–Lux construct was provided by J. Massagué. This work was supported by the Erna D. Leir Foundation for Research of Degenerative Brain Diseases, the National Alliance for Research of Schizophrenia and Depression, the National Parkinson Foundation Inc., the Israel Science Foundation funded by the Israel Academy of Sciences and Humanities (J.O.), and the NIH. H.S. is an investigator of the Howard Hughes Medical Institute. Correspondence and requests for materials should be addressed to A.B.R.
ASJC Scopus subject areas
- Cell Biology