TY - JOUR
T1 - A new N-Acyl homoserine lactone synthase in an uncultured symbiont of the red sea sponge Theonella swinhoei
AU - Britstein, Maya
AU - Devescovi, Giulia
AU - Handley, Kim M.
AU - Malik, Assaf
AU - Haber, Markus
AU - Saurav, Kumar
AU - Teta, Roberta
AU - Costantino, Valeria
AU - Burgsdorf, Ilia
AU - Gilbert, Jack A.
AU - Sher, Noa
AU - Venturi, Vittorio
AU - Steindler, Laura
N1 - Publisher Copyright:
© 2016, American Society for Microbiology.
PY - 2016
Y1 - 2016
N2 - Sponges harbor a remarkable diversity of microbial symbionts in which signal molecules can accumulate and enable cell-cell communication, such as quorum sensing (QS). Bacteria capable of QS were isolated from marine sponges; however, an extremely small fraction of the sponge microbiome is amenable to cultivation. We took advantage of community genome assembly and binning to investigate the uncultured majority of sponge symbionts. We identified a complete N-acyl-homoserine lactone (AHL)-QS system (designated TswIR) and seven partial luxI homologues in the microbiome of Theonella swinhoei. The TswIR system was novel and shown to be associated with an alphaproteobacterium of the order Rhodobacterales, here termed Rhodobacterales bacterium TS309. The tswI gene, when expressed in Escherichia coli, produced three AHLs, two of which were also identified in a T. swinhoei sponge extract. The taxonomic affiliation of the 16S rRNA of Rhodobacterales bacterium TS309 to a sponge-coral specific clade, its enrichment in sponge versus seawater and marine sediment samples, and the presence of spongespecific features, such as ankyrin-like domains and tetratricopeptide repeats, indicate a likely symbiotic nature of this bacterium.
AB - Sponges harbor a remarkable diversity of microbial symbionts in which signal molecules can accumulate and enable cell-cell communication, such as quorum sensing (QS). Bacteria capable of QS were isolated from marine sponges; however, an extremely small fraction of the sponge microbiome is amenable to cultivation. We took advantage of community genome assembly and binning to investigate the uncultured majority of sponge symbionts. We identified a complete N-acyl-homoserine lactone (AHL)-QS system (designated TswIR) and seven partial luxI homologues in the microbiome of Theonella swinhoei. The TswIR system was novel and shown to be associated with an alphaproteobacterium of the order Rhodobacterales, here termed Rhodobacterales bacterium TS309. The tswI gene, when expressed in Escherichia coli, produced three AHLs, two of which were also identified in a T. swinhoei sponge extract. The taxonomic affiliation of the 16S rRNA of Rhodobacterales bacterium TS309 to a sponge-coral specific clade, its enrichment in sponge versus seawater and marine sediment samples, and the presence of spongespecific features, such as ankyrin-like domains and tetratricopeptide repeats, indicate a likely symbiotic nature of this bacterium.
UR - http://www.scopus.com/inward/record.url?scp=84957900140&partnerID=8YFLogxK
U2 - 10.1128/AEM.03111-15
DO - 10.1128/AEM.03111-15
M3 - Article
C2 - 26655754
AN - SCOPUS:84957900140
SN - 0099-2240
VL - 82
SP - 1274
EP - 1285
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
IS - 4
ER -