A new approach for cultivating the cyanobacterium Nostoc calcicola (MACC-612) to produce biomass and bioactive compounds using a thin-layer raceway pond

Paula S.M. Celis-Plá; Tomás Agustín Rearte; Amir Neori; Jiří Masojídek; José Bonomi-Barufi; Félix Álvarez-Gómez; Karolína Ranglová; Jaqueline Carmo da Silva; Roberto Abdala; Cintia Gómez; Martín Caporgno; Giuseppe Torzillo; Ana Margarita Silva Benavides; Peter J. Ralph; Thaís Fávero Massocato; Richard Atzmüller; Julia Vega; Patricia Chávez; Félix L. Figueroa

doi:10.1016/j.algal.2021.102421

A new approach for cultivating the cyanobacterium Nostoc calcicola (MACC-612) to produce biomass and bioactive compounds using a thin-layer raceway pond

Paula S.M. Celis-Plá, Tomás Agustín Rearte, Amir Neori, Jiří Masojídek, José Bonomi-Barufi, Félix Álvarez-Gómez, Karolína Ranglová, Jaqueline Carmo da Silva, Roberto Abdala, Cintia Gómez, Martín Caporgno, Giuseppe Torzillo, Ana Margarita Silva Benavides, Peter J. Ralph, Thaís Fávero Massocato, Richard Atzmüller, Julia Vega, Patricia Chávez, Félix L. Figueroa

Department of Marine Biology

Research output: Contribution to journal › Article › peer-review

Abstract

The culture of microalgae and cyanobacteria in open systems has been improved through the novel approach of thin-layer raceway ponds. The importance of studying mass cultivation of the cyanobacterium Nostoc calcicola (further as Nostoc) lies in its biotechnological potential as a source of bioactive compounds for food and non-food applications. These compounds include polysaccharides, mycosporine-like amino acids and phycocyanin. Nostoc was cultured outdoors in a thin-layer raceway pond where the biomass production, physiological status, photosynthetic activity, and biochemical composition were monitored through the experimental period of 5 days. The biomass, as did the maximal quantum yield of PSII, maximal electron transport rate (ETR_max) and photosynthetic efficiency (α_ETR) increased throughout the experimental period showing the optimal operation of the thin-layer raceway ponds, due to the light penetrates deeper into the thin culture layer and thus more light is available to the cells. Oxygen levels in the culture increased over time, but no photoinhibition was evident indicating optimal action of non-photochemical mechanisms. Nostoc increased the total internal carbon content over the experimental period. Chlorophyll increased, whereas the N compounds such as the biliprotein phycocyanin decreased. Among the UV-absorbing compounds, polyphenols, mycosporine-like amino acids, such as shinorine and other unknown UV-A absorbing compounds were detected. There components showed a positive correlation to antioxidant activity. Thus, the optimal accumulation of biomass and the accumulation of bio-active compounds having antioxidant capacity show the possible biotechnological applications of Nostoc.

Original language	English
Article number	102421
Journal	Algal Research
Volume	59
DOIs	https://doi.org/10.1016/j.algal.2021.102421
State	Published - Nov 2021

Bibliographical note

Funding Information:
The authors thank Ms. Soňa Pekařová, Mr. Jan Pilný, Mr. Michal Bureš and Mr. Petr Novotný for technical assistance and Dr. Richard Lhotský for experiment management. The authors thank to the local organizers of Algatech Center of the Institute of Microbiology (Czech Academic of Sciences) the organization of GAP10 workshop “Aquatic productivity - in the Omics Era”, held in Třeboň (Czech Republic) between 19 and 30 August 2017. This work was supported by the EU program Horizon 2020 (project SABANA, grant no. 727874) and in part by the Program INTERREG V-A Austria – Czech Republic, project ATCZ221 Algae 4 Fish. PSMCP was supported by the Santander scholarship to young research 2017 and project FONDECYT N° 11180197. FLF thanks Junta de Andalucía for financial support of the research group “Photobiology and biotechnology of aquatic organisms” (FYBOA-RNM295). TAR was supported by the Universidad de Buenos Aires, Argentina. AN thanks BARD Research Grant Award No. US – 4599-13R (The United States – Israel Binational Agricultural Research and Development Fund) and additional funds from the Israeli Ministry for Science and Technology and the Dead Sea-Arava Science Center (DSASC) to AN. This special issue of Algal Research is dedicated to Prof. Jacco Kromkamp, regular participant and organizer of GAP workshops, who unluckily passed away in October 2020.

Funding Information:
This work was supported by the EU program Horizon 2020 (project SABANA, grant no. 727874 ) and in part by the Program INTERREG V-A Austria – Czech Republic , project ATCZ221 Algae 4 Fish. PSMCP was supported by the Santander scholarship to young research 2017 and project FONDECYT N° 11180197 . FLF thanks Junta de Andalucía for financial support of the research group “Photobiology and biotechnology of aquatic organisms” ( FYBOA-RNM295 ). TAR was supported by the Universidad de Buenos Aires , Argentina. AN thanks BARD Research Grant Award No. US – 4599-13R (The United States – Israel Binational Agricultural Research and Development Fund) and additional funds from the Israeli Ministry for Science and Technology and the Dead Sea-Arava Science Center (DSASC) to AN.

Publisher Copyright:
© 2021 Elsevier B.V.

Keywords

Aquaculture
Biochemical composition
In-vivo chlorophyll a fluorescence
Nostoc
Photosynthesis
Thin-layer raceway cultivator

ASJC Scopus subject areas

Agronomy and Crop Science

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10.1016/j.algal.2021.102421

Cite this

Celis-Plá, P. S. M., Rearte, T. A., Neori, A., Masojídek, J., Bonomi-Barufi, J., Álvarez-Gómez, F., Ranglová, K., Carmo da Silva, J., Abdala, R., Gómez, C., Caporgno, M., Torzillo, G., Silva Benavides, A. M., Ralph, P. J., Fávero Massocato, T., Atzmüller, R., Vega, J., Chávez, P., & Figueroa, F. L. (2021). A new approach for cultivating the cyanobacterium Nostoc calcicola (MACC-612) to produce biomass and bioactive compounds using a thin-layer raceway pond. Algal Research, 59, Article 102421. https://doi.org/10.1016/j.algal.2021.102421

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title = "A new approach for cultivating the cyanobacterium Nostoc calcicola (MACC-612) to produce biomass and bioactive compounds using a thin-layer raceway pond",

abstract = "The culture of microalgae and cyanobacteria in open systems has been improved through the novel approach of thin-layer raceway ponds. The importance of studying mass cultivation of the cyanobacterium Nostoc calcicola (further as Nostoc) lies in its biotechnological potential as a source of bioactive compounds for food and non-food applications. These compounds include polysaccharides, mycosporine-like amino acids and phycocyanin. Nostoc was cultured outdoors in a thin-layer raceway pond where the biomass production, physiological status, photosynthetic activity, and biochemical composition were monitored through the experimental period of 5 days. The biomass, as did the maximal quantum yield of PSII, maximal electron transport rate (ETRmax) and photosynthetic efficiency (αETR) increased throughout the experimental period showing the optimal operation of the thin-layer raceway ponds, due to the light penetrates deeper into the thin culture layer and thus more light is available to the cells. Oxygen levels in the culture increased over time, but no photoinhibition was evident indicating optimal action of non-photochemical mechanisms. Nostoc increased the total internal carbon content over the experimental period. Chlorophyll increased, whereas the N compounds such as the biliprotein phycocyanin decreased. Among the UV-absorbing compounds, polyphenols, mycosporine-like amino acids, such as shinorine and other unknown UV-A absorbing compounds were detected. There components showed a positive correlation to antioxidant activity. Thus, the optimal accumulation of biomass and the accumulation of bio-active compounds having antioxidant capacity show the possible biotechnological applications of Nostoc.",

keywords = "Aquaculture, Biochemical composition, In-vivo chlorophyll a fluorescence, Nostoc, Photosynthesis, Thin-layer raceway cultivator",

author = "Celis-Pl{\'a}, {Paula S.M.} and Rearte, {Tom{\'a}s Agust{\'i}n} and Amir Neori and Ji{\v r}{\'i} Masoj{\'i}dek and Jos{\'e} Bonomi-Barufi and F{\'e}lix {\'A}lvarez-G{\'o}mez and Karol{\'i}na Ranglov{\'a} and {Carmo da Silva}, Jaqueline and Roberto Abdala and Cintia G{\'o}mez and Mart{\'i}n Caporgno and Giuseppe Torzillo and {Silva Benavides}, {Ana Margarita} and Ralph, {Peter J.} and {F{\'a}vero Massocato}, Tha{\'i}s and Richard Atzm{\"u}ller and Julia Vega and Patricia Ch{\'a}vez and Figueroa, {F{\'e}lix L.}",

note = "Funding Information: The authors thank Ms. So{\v n}a Peka{\v r}ov{\'a}, Mr. Jan Piln{\'y}, Mr. Michal Bure{\v s} and Mr. Petr Novotn{\'y} for technical assistance and Dr. Richard Lhotsk{\'y} for experiment management. The authors thank to the local organizers of Algatech Center of the Institute of Microbiology (Czech Academic of Sciences) the organization of GAP10 workshop “Aquatic productivity - in the Omics Era”, held in T{\v r}ebo{\v n} (Czech Republic) between 19 and 30 August 2017. This work was supported by the EU program Horizon 2020 (project SABANA, grant no. 727874) and in part by the Program INTERREG V-A Austria – Czech Republic, project ATCZ221 Algae 4 Fish. PSMCP was supported by the Santander scholarship to young research 2017 and project FONDECYT N° 11180197. FLF thanks Junta de Andaluc{\'i}a for financial support of the research group “Photobiology and biotechnology of aquatic organisms” (FYBOA-RNM295). TAR was supported by the Universidad de Buenos Aires, Argentina. AN thanks BARD Research Grant Award No. US – 4599-13R (The United States – Israel Binational Agricultural Research and Development Fund) and additional funds from the Israeli Ministry for Science and Technology and the Dead Sea-Arava Science Center (DSASC) to AN. This special issue of Algal Research is dedicated to Prof. Jacco Kromkamp, regular participant and organizer of GAP workshops, who unluckily passed away in October 2020. Funding Information: This work was supported by the EU program Horizon 2020 (project SABANA, grant no. 727874 ) and in part by the Program INTERREG V-A Austria – Czech Republic , project ATCZ221 Algae 4 Fish. PSMCP was supported by the Santander scholarship to young research 2017 and project FONDECYT N° 11180197 . FLF thanks Junta de Andaluc{\'i}a for financial support of the research group “Photobiology and biotechnology of aquatic organisms” ( FYBOA-RNM295 ). TAR was supported by the Universidad de Buenos Aires , Argentina. AN thanks BARD Research Grant Award No. US – 4599-13R (The United States – Israel Binational Agricultural Research and Development Fund) and additional funds from the Israeli Ministry for Science and Technology and the Dead Sea-Arava Science Center (DSASC) to AN. Publisher Copyright: {\textcopyright} 2021 Elsevier B.V.",

year = "2021",

month = nov,

doi = "10.1016/j.algal.2021.102421",

language = "English",

volume = "59",

journal = "Algal Research",

issn = "2211-9264",

publisher = "Elsevier",

}

Celis-Plá, PSM, Rearte, TA, Neori, A, Masojídek, J, Bonomi-Barufi, J, Álvarez-Gómez, F, Ranglová, K, Carmo da Silva, J, Abdala, R, Gómez, C, Caporgno, M, Torzillo, G, Silva Benavides, AM, Ralph, PJ, Fávero Massocato, T, Atzmüller, R, Vega, J, Chávez, P & Figueroa, FL 2021, 'A new approach for cultivating the cyanobacterium Nostoc calcicola (MACC-612) to produce biomass and bioactive compounds using a thin-layer raceway pond', Algal Research, vol. 59, 102421. https://doi.org/10.1016/j.algal.2021.102421

TY - JOUR

T1 - A new approach for cultivating the cyanobacterium Nostoc calcicola (MACC-612) to produce biomass and bioactive compounds using a thin-layer raceway pond

AU - Celis-Plá, Paula S.M.

AU - Rearte, Tomás Agustín

AU - Neori, Amir

AU - Masojídek, Jiří

AU - Bonomi-Barufi, José

AU - Álvarez-Gómez, Félix

AU - Ranglová, Karolína

AU - Carmo da Silva, Jaqueline

AU - Abdala, Roberto

AU - Gómez, Cintia

AU - Caporgno, Martín

AU - Torzillo, Giuseppe

AU - Silva Benavides, Ana Margarita

AU - Ralph, Peter J.

AU - Fávero Massocato, Thaís

AU - Atzmüller, Richard

AU - Vega, Julia

AU - Chávez, Patricia

AU - Figueroa, Félix L.

N1 - Funding Information: The authors thank Ms. Soňa Pekařová, Mr. Jan Pilný, Mr. Michal Bureš and Mr. Petr Novotný for technical assistance and Dr. Richard Lhotský for experiment management. The authors thank to the local organizers of Algatech Center of the Institute of Microbiology (Czech Academic of Sciences) the organization of GAP10 workshop “Aquatic productivity - in the Omics Era”, held in Třeboň (Czech Republic) between 19 and 30 August 2017. This work was supported by the EU program Horizon 2020 (project SABANA, grant no. 727874) and in part by the Program INTERREG V-A Austria – Czech Republic, project ATCZ221 Algae 4 Fish. PSMCP was supported by the Santander scholarship to young research 2017 and project FONDECYT N° 11180197. FLF thanks Junta de Andalucía for financial support of the research group “Photobiology and biotechnology of aquatic organisms” (FYBOA-RNM295). TAR was supported by the Universidad de Buenos Aires, Argentina. AN thanks BARD Research Grant Award No. US – 4599-13R (The United States – Israel Binational Agricultural Research and Development Fund) and additional funds from the Israeli Ministry for Science and Technology and the Dead Sea-Arava Science Center (DSASC) to AN. This special issue of Algal Research is dedicated to Prof. Jacco Kromkamp, regular participant and organizer of GAP workshops, who unluckily passed away in October 2020. Funding Information: This work was supported by the EU program Horizon 2020 (project SABANA, grant no. 727874 ) and in part by the Program INTERREG V-A Austria – Czech Republic , project ATCZ221 Algae 4 Fish. PSMCP was supported by the Santander scholarship to young research 2017 and project FONDECYT N° 11180197 . FLF thanks Junta de Andalucía for financial support of the research group “Photobiology and biotechnology of aquatic organisms” ( FYBOA-RNM295 ). TAR was supported by the Universidad de Buenos Aires , Argentina. AN thanks BARD Research Grant Award No. US – 4599-13R (The United States – Israel Binational Agricultural Research and Development Fund) and additional funds from the Israeli Ministry for Science and Technology and the Dead Sea-Arava Science Center (DSASC) to AN. Publisher Copyright: © 2021 Elsevier B.V.

PY - 2021/11

Y1 - 2021/11

N2 - The culture of microalgae and cyanobacteria in open systems has been improved through the novel approach of thin-layer raceway ponds. The importance of studying mass cultivation of the cyanobacterium Nostoc calcicola (further as Nostoc) lies in its biotechnological potential as a source of bioactive compounds for food and non-food applications. These compounds include polysaccharides, mycosporine-like amino acids and phycocyanin. Nostoc was cultured outdoors in a thin-layer raceway pond where the biomass production, physiological status, photosynthetic activity, and biochemical composition were monitored through the experimental period of 5 days. The biomass, as did the maximal quantum yield of PSII, maximal electron transport rate (ETRmax) and photosynthetic efficiency (αETR) increased throughout the experimental period showing the optimal operation of the thin-layer raceway ponds, due to the light penetrates deeper into the thin culture layer and thus more light is available to the cells. Oxygen levels in the culture increased over time, but no photoinhibition was evident indicating optimal action of non-photochemical mechanisms. Nostoc increased the total internal carbon content over the experimental period. Chlorophyll increased, whereas the N compounds such as the biliprotein phycocyanin decreased. Among the UV-absorbing compounds, polyphenols, mycosporine-like amino acids, such as shinorine and other unknown UV-A absorbing compounds were detected. There components showed a positive correlation to antioxidant activity. Thus, the optimal accumulation of biomass and the accumulation of bio-active compounds having antioxidant capacity show the possible biotechnological applications of Nostoc.

AB - The culture of microalgae and cyanobacteria in open systems has been improved through the novel approach of thin-layer raceway ponds. The importance of studying mass cultivation of the cyanobacterium Nostoc calcicola (further as Nostoc) lies in its biotechnological potential as a source of bioactive compounds for food and non-food applications. These compounds include polysaccharides, mycosporine-like amino acids and phycocyanin. Nostoc was cultured outdoors in a thin-layer raceway pond where the biomass production, physiological status, photosynthetic activity, and biochemical composition were monitored through the experimental period of 5 days. The biomass, as did the maximal quantum yield of PSII, maximal electron transport rate (ETRmax) and photosynthetic efficiency (αETR) increased throughout the experimental period showing the optimal operation of the thin-layer raceway ponds, due to the light penetrates deeper into the thin culture layer and thus more light is available to the cells. Oxygen levels in the culture increased over time, but no photoinhibition was evident indicating optimal action of non-photochemical mechanisms. Nostoc increased the total internal carbon content over the experimental period. Chlorophyll increased, whereas the N compounds such as the biliprotein phycocyanin decreased. Among the UV-absorbing compounds, polyphenols, mycosporine-like amino acids, such as shinorine and other unknown UV-A absorbing compounds were detected. There components showed a positive correlation to antioxidant activity. Thus, the optimal accumulation of biomass and the accumulation of bio-active compounds having antioxidant capacity show the possible biotechnological applications of Nostoc.

KW - Aquaculture

KW - Biochemical composition

KW - In-vivo chlorophyll a fluorescence

KW - Nostoc

KW - Photosynthesis

KW - Thin-layer raceway cultivator

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U2 - 10.1016/j.algal.2021.102421

DO - 10.1016/j.algal.2021.102421

M3 - Article

AN - SCOPUS:85111704098

SN - 2211-9264

VL - 59

JO - Algal Research

JF - Algal Research

M1 - 102421

ER -

A new approach for cultivating the cyanobacterium Nostoc calcicola (MACC-612) to produce biomass and bioactive compounds using a thin-layer raceway pond

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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