A Multireceptor Genetic Approach Uncovers an Ordered Integration of VNO Sensory Inputs in the Accessory Olfactory Bulb

Shlomo Wagner; Amy L. Gresser; A. Thomas Torello; Catherine Dulac

doi:10.1016/j.neuron.2006.04.033

A Multireceptor Genetic Approach Uncovers an Ordered Integration of VNO Sensory Inputs in the Accessory Olfactory Bulb

Shlomo Wagner, Amy L. Gresser, A. Thomas Torello, Catherine Dulac

Research output: Contribution to journal › Article › peer-review

Abstract

Pheromone detection by the vomeronasal organ (VNO) is thought to rely on activation of specific receptors from the V1R and V2R gene families, but the central representation of pheromone receptor activation remains poorly understood. We generated transgenic mouse lines in which projections from multiple populations of VNO neurons, each expressing a distinct V1R, are differentially labeled with fluorescent proteins. This approach revealed that inputs from neurons expressing closely related V1Rs intermingle within shared, spatially conserved domains of the accessory olfactory bulb (AOB). Mitral cell-glomerular connectivity was examined by injecting intracellular dyes into AOB mitral cells and monitoring dendritic contacts with genetically labeled glomeruli. We show that individual mitral cells extend dendrites to glomeruli associated with different, but likely closely related, V1Rs. This organization differs from the labeled line of OR signaling in the main olfactory system and suggests that integration of information may already occur at the level of the AOB.

Original language	English
Pages (from-to)	697-709
Number of pages	13
Journal	Neuron
Volume	50
Issue number	5
DOIs	https://doi.org/10.1016/j.neuron.2006.04.033
State	Published - 1 Jun 2006
Externally published	Yes

Bibliographical note

Funding Information:
We thank R. Hellmiss for artistic work, J. Dubauskaite for mouse transgenics, Drs. J. Lichtman and J. Tapia for advice on imaging techniques, Dr. N. Book for excellent technical assistance, Drs. Y. Yarom and A. Mizrahi for their generous help, and the Dulac lab for helpful discussions and comments on the manuscript. This work was supported by the Howard Hughes Medical Institute (C.D.), the National Institute for Psychobiology in Israel (S.W.), the Interdisciplinary Center for Computational Neuroscience of the Hebrew University, Jerusalem (S.W.), NIH-NIDCD grant R01 DC003903 (C.D.), and EMBO long-term fellowship # 283-1998 (S.W.).

Keywords

CELLBIO
MOLNEURO

ASJC Scopus subject areas

General Neuroscience

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10.1016/j.neuron.2006.04.033

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title = "A Multireceptor Genetic Approach Uncovers an Ordered Integration of VNO Sensory Inputs in the Accessory Olfactory Bulb",

abstract = "Pheromone detection by the vomeronasal organ (VNO) is thought to rely on activation of specific receptors from the V1R and V2R gene families, but the central representation of pheromone receptor activation remains poorly understood. We generated transgenic mouse lines in which projections from multiple populations of VNO neurons, each expressing a distinct V1R, are differentially labeled with fluorescent proteins. This approach revealed that inputs from neurons expressing closely related V1Rs intermingle within shared, spatially conserved domains of the accessory olfactory bulb (AOB). Mitral cell-glomerular connectivity was examined by injecting intracellular dyes into AOB mitral cells and monitoring dendritic contacts with genetically labeled glomeruli. We show that individual mitral cells extend dendrites to glomeruli associated with different, but likely closely related, V1Rs. This organization differs from the labeled line of OR signaling in the main olfactory system and suggests that integration of information may already occur at the level of the AOB.",

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author = "Shlomo Wagner and Gresser, {Amy L.} and Torello, {A. Thomas} and Catherine Dulac",

note = "Funding Information: We thank R. Hellmiss for artistic work, J. Dubauskaite for mouse transgenics, Drs. J. Lichtman and J. Tapia for advice on imaging techniques, Dr. N. Book for excellent technical assistance, Drs. Y. Yarom and A. Mizrahi for their generous help, and the Dulac lab for helpful discussions and comments on the manuscript. This work was supported by the Howard Hughes Medical Institute (C.D.), the National Institute for Psychobiology in Israel (S.W.), the Interdisciplinary Center for Computational Neuroscience of the Hebrew University, Jerusalem (S.W.), NIH-NIDCD grant R01 DC003903 (C.D.), and EMBO long-term fellowship # 283-1998 (S.W.). ",

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A Multireceptor Genetic Approach Uncovers an Ordered Integration of VNO Sensory Inputs in the Accessory Olfactory Bulb

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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