A high molecular weight cytotoxic lymphokine in supernatants of lymphokine-activated killer cells cross-reacts with transforming growth factor beta

Sarit Larisch-Bloch; Barbara Sterman; Dov Sulitzeanu

doi:10.1016/0165-2478(90)90144-F

A high molecular weight cytotoxic lymphokine in supernatants of lymphokine-activated killer cells cross-reacts with transforming growth factor beta

Sarit Larisch-Bloch, Barbara Sterman, Dov Sulitzeanu

Research output: Contribution to journal › Article › peer-review

Abstract

Supernatants of lymphokine-activated killer (LAK) cells were highly cytotoxic for melanoma A375 cells. A high-molecular-weight fraction was isolated from such supernatants by gel filtration on an S-300 Sephacryl column (Fraction 1; Fr1). The cytotoxic activity in Fr1 was heat- and acid-resistant and was completely abolished by a rabbit antibody against TGF-β. We conclude that Fr1 contains TGF-β or a cross-reactive molecule, associated with a high-molecular-weight carrier.

Original language	English
Pages (from-to)	189-192
Number of pages	4
Journal	Immunology Letters
Volume	26
Issue number	2
DOIs	https://doi.org/10.1016/0165-2478(90)90144-F
State	Published - Nov 1990
Externally published	Yes

Keywords

Cross-reaction with TGF-β
Cytotoxic supernatant
Lymphokine-activated killer cell

ASJC Scopus subject areas

Immunology and Allergy
Immunology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0165-2478(90)90144-F

Cite this

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title = "A high molecular weight cytotoxic lymphokine in supernatants of lymphokine-activated killer cells cross-reacts with transforming growth factor beta",

abstract = "Supernatants of lymphokine-activated killer (LAK) cells were highly cytotoxic for melanoma A375 cells. A high-molecular-weight fraction was isolated from such supernatants by gel filtration on an S-300 Sephacryl column (Fraction 1; Fr1). The cytotoxic activity in Fr1 was heat- and acid-resistant and was completely abolished by a rabbit antibody against TGF-β. We conclude that Fr1 contains TGF-β or a cross-reactive molecule, associated with a high-molecular-weight carrier.",

keywords = "Cross-reaction with TGF-β, Cytotoxic supernatant, Lymphokine-activated killer cell",

author = "Sarit Larisch-Bloch and Barbara Sterman and Dov Sulitzeanu",

year = "1990",

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doi = "10.1016/0165-2478(90)90144-F",

language = "English",

volume = "26",

pages = "189--192",

journal = "Immunology Letters",

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T1 - A high molecular weight cytotoxic lymphokine in supernatants of lymphokine-activated killer cells cross-reacts with transforming growth factor beta

AU - Larisch-Bloch, Sarit

AU - Sterman, Barbara

AU - Sulitzeanu, Dov

PY - 1990/11

Y1 - 1990/11

N2 - Supernatants of lymphokine-activated killer (LAK) cells were highly cytotoxic for melanoma A375 cells. A high-molecular-weight fraction was isolated from such supernatants by gel filtration on an S-300 Sephacryl column (Fraction 1; Fr1). The cytotoxic activity in Fr1 was heat- and acid-resistant and was completely abolished by a rabbit antibody against TGF-β. We conclude that Fr1 contains TGF-β or a cross-reactive molecule, associated with a high-molecular-weight carrier.

AB - Supernatants of lymphokine-activated killer (LAK) cells were highly cytotoxic for melanoma A375 cells. A high-molecular-weight fraction was isolated from such supernatants by gel filtration on an S-300 Sephacryl column (Fraction 1; Fr1). The cytotoxic activity in Fr1 was heat- and acid-resistant and was completely abolished by a rabbit antibody against TGF-β. We conclude that Fr1 contains TGF-β or a cross-reactive molecule, associated with a high-molecular-weight carrier.

KW - Cross-reaction with TGF-β

KW - Cytotoxic supernatant

KW - Lymphokine-activated killer cell

UR - http://www.scopus.com/inward/record.url?scp=0025107333&partnerID=8YFLogxK

U2 - 10.1016/0165-2478(90)90144-F

DO - 10.1016/0165-2478(90)90144-F

M3 - Article

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AN - SCOPUS:0025107333

SN - 0165-2478

VL - 26

SP - 189

EP - 192

JO - Immunology Letters

JF - Immunology Letters

IS - 2

ER -

A high molecular weight cytotoxic lymphokine in supernatants of lymphokine-activated killer cells cross-reacts with transforming growth factor beta

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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