Supernatants of lymphokine-activated killer (LAK) cells were highly cytotoxic for melanoma A375 cells. A high-molecular-weight fraction was isolated from such supernatants by gel filtration on an S-300 Sephacryl column (Fraction 1; Fr1). The cytotoxic activity in Fr1 was heat- and acid-resistant and was completely abolished by a rabbit antibody against TGF-β. We conclude that Fr1 contains TGF-β or a cross-reactive molecule, associated with a high-molecular-weight carrier.
- Cross-reaction with TGF-β
- Cytotoxic supernatant
- Lymphokine-activated killer cell
ASJC Scopus subject areas
- Immunology and Allergy