A CNL protein in wild emmer wheat confers powdery mildew resistance

Miaomiao Li, Lingli Dong, Beibei Li, Zhengzhong Wang, Jingzhong Xie, Dan Qiu, Yahui Li, Wenqi Shi, Lijun Yang, Qiuhong Wu, Yongxing Chen, Ping Lu, Guanghao Guo, Huaizhi Zhang, Panpan Zhang, Keyu Zhu, Yiwen Li, Yan Zhang, Rongge Wang, Chengguo YuanWei Liu, Dazhao Yu, Ming Cheng Luo, Tzion Fahima, Eviatar Nevo, Hongjie Li, Zhiyong Liu

Research output: Contribution to journalArticlepeer-review

Abstract

Powdery mildew, a fungal disease caused by Blumeria graminis f. sp. tritici (Bgt), has a serious impact on wheat production. Loss of resistance in cultivars prompts a continuing search for new sources of resistance. Wild emmer wheat (Triticum turgidum ssp. dicoccoides, WEW), the progenitor of both modern tetraploid and hexaploid wheats, harbors many powdery mildew resistance genes. We report here the positional cloning and functional characterization of Pm41, a powdery mildew resistance gene derived from WEW, which encodes a coiled-coil, nucleotide-binding site and leucine-rich repeat protein (CNL). Mutagenesis and stable genetic transformation confirmed the function of Pm41 against Bgt infection in wheat. We demonstrated that Pm41 was present at a very low frequency (1.81%) only in southern WEW populations. It was absent in other WEW populations, domesticated emmer, durum, and common wheat, suggesting that the ancestral Pm41 was restricted to its place of origin and was not incorporated into domesticated wheat. Our findings emphasize the importance of conservation and exploitation of the primary WEW gene pool, as a valuable resource for discovery of resistance genes for improvement of modern wheat cultivars.

Original languageEnglish
Pages (from-to)1027-1037
Number of pages11
JournalNew Phytologist
Volume228
Issue number3
DOIs
StatePublished - 1 Nov 2020

Bibliographical note

Funding Information:
We are grateful to Profs Qixin Sun and Tsomin Yang, China Agricultural University, for their advice and support during the research. Many thanks to Drs Xueyong Zhang, Xianchun Xia and Lihui Li, Chinese Academy of Agricultural Sciences, Beijing, for providing the Chinese wheat mini‐core collection (MCC), worldwide wheat landrace collection, and Chinese wheat landrace collection, respectively. We appreciate the assistance of Dr Genying Li of Shandong Academy of Agriculture Sciences, Jinan, in developing the transgenic wheat materials. This study was supported by the grants from the National Key Research and Development Program of China (2016YFD0101802), National Natural Science Foundation of China (30425039, 31030056), The Agriculture Science and Technology Innovation Program, and Science and Technology Service Network Initiative of Chinese Academy of Sciences (KFJ‐STS‐ZDTP‐024). The authors declare that they have no conflicts of interest.

Funding Information:
We are grateful to Profs Qixin Sun and Tsomin Yang, China Agricultural University, for their advice and support during the research. Many thanks to Drs Xueyong Zhang, Xianchun Xia and Lihui Li, Chinese Academy of Agricultural Sciences, Beijing, for providing the Chinese wheat mini-core collection (MCC), worldwide wheat landrace collection, and Chinese wheat landrace collection, respectively. We appreciate the assistance of Dr Genying Li of Shandong Academy of Agriculture Sciences, Jinan, in developing the transgenic wheat materials. This study was supported by the grants from the National Key Research and Development Program of China (2016YFD0101802), National Natural Science Foundation of China (30425039, 31030056), The Agriculture Science and Technology Innovation Program, and Science and Technology Service Network Initiative of Chinese Academy of Sciences (KFJ-STS-ZDTP-024). The authors declare that they have no conflicts of interest.

Publisher Copyright:
© 2020 The Authors. New Phytologist © 2020 New Phytologist Trust

Keywords

  • Blumeria graminis f. sp. tritici
  • Pm41
  • Triticum turgidum ssp. dicoccoides
  • coiled-coil–nucleotide-binding site–leucine-rich repeat (CC-NBS-LRR)
  • positional cloning

ASJC Scopus subject areas

  • Physiology
  • Plant Science

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