Abstract
Soybean cyst nematode (Heterodera glycines Ichinohe) is one of the most economically destructive pathogens worldwide. The cultivation of resistant soybean cultivars combined with crop rotation was the most effective management strategy to reduce yield losses due to SCN. To accelerate the breeding of cultivars resistant to SCN under molecular marker assisted selection, we developed an economical, time-saving and high-throughput KASP marker GmSNAP11-5149 by using a reported non-synonymous polymorphism (Map-5149) on the minor SCN3-11 resistant to SCN3. Two hundred and two soybean accessions from eight countries with known SCN3 were genotyped for GmSNAP11-5149. The average female index of 141 accessions carrying GmSNAP11-5149-AA (6.2%) was significantly lower than that of 58 accessions carrying GmSNAP11-5149-GG (61.1%) at the P < 0.0001 probability level. The selection efficiency of GmSNAP11-5149 for resistant accessions was 92%. It is suggested that GmSNAP11-5149 could be used to effectively distinguish resistant genotypes from susceptible ones in the tested soybean accessions and subsequently could be used in molecular marker-assisted breeding and identification of resistant germplasm.
| Translated title of the contribution | Development and Utilization of KASP Marker for SCN3-11 Locus Resistant to Soybean Cyst Nematode |
|---|---|
| Original language | Chinese (Simplified) |
| Pages (from-to) | 1600-1611 |
| Number of pages | 12 |
| Journal | Acta Agronomica Sinica(China) |
| Volume | 44 |
| Issue number | 11 |
| DOIs | |
| State | Published - 12 Nov 2018 |
| Externally published | Yes |
Bibliographical note
Publisher Copyright:© 2018, Science Press. All right reserved.
Keywords
- KASP marker
- Marker-assisted selection (MAS)
- Soybean cyst nematode
ASJC Scopus subject areas
- Biotechnology
- Agronomy and Crop Science
- Plant Science
