Abstract
Zhongpin 03-5373 with resistance to SCN3 is an elite soybean line, which is traced back to 10 parental lines. Three elite resistant sources (Huipizhiheidou, Peking, and PI437654), diversely used in the soybean cyst nematode resistant breeding, were included in the pedigree of Zhongpin 03-5373. In this study, 152 molecular markers were selected to detect polymorphism among the 11 materials. A total of 437 alleles were identified with average of 2.9, ranging from two to five alleles on each locus. The average of Nei’s genetic identity among pairwise cultivars were 0.458, the haplotypes formed by four SSR markers (Satt152, Satt179, Barcsoyssr_18_107, and Satt196) could distinguish 11 cultivars in this study. Pedigree tracing elucidated genomic variation in breeding. IBD analysis showed that Huipizhiheidou contributed the most of specific alleles (39) to Zhongpin 03-5373 among three resistant genetic resources (Huipizhiheidou, Peking, and PI437654), followed by PI437654 (6). In addition, 20 markers identified were related to the resistance to SCN 3. These results supplied the information for further cloning resistant gene and resistant breeding by marker assistant selection.
| Translated title of the contribution | Genetic Dissection of Elite Line Zhongpin 03-5373 Pedigree and Identification of Candidate Markers Related to Resistance to Soybean Cyst Nematode |
|---|---|
| Original language | Chinese (Simplified) |
| Pages (from-to) | 1746-1753 |
| Number of pages | 8 |
| Journal | Acta Agronomica Sinica(China) |
| Volume | 39 |
| Issue number | 10 |
| DOIs | |
| State | Published - 12 Oct 2013 |
| Externally published | Yes |
Bibliographical note
Publisher Copyright:© 2013 Institute of Crop Sciences. All rights reserved.
Keywords
- Pedigree analysis, Molecular marker
- Soybean
- Soybean cyst nematode
ASJC Scopus subject areas
- Biotechnology
- Agronomy and Crop Science
- Plant Science